Cloned (Comment) | Organism |
---|---|
gene glmU, recombinant expression of His-tagged enzyme in Escherichia coli strain BL12(DE3), overexpression of GlmU in Mycobacterium tuberculosis | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of Mycobacterium tuberculosis mutant strains overproducing GlmU allows determination of the contribution of the protein to mycobacterial entry into human neutrophils | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | Mycobacterium tuberculosis | - |
diphosphate + UDP-N-acetyl-alpha-D-glucosamine | - |
? | |
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | Mycobacterium tuberculosis H37Rv | - |
diphosphate + UDP-N-acetyl-alpha-D-glucosamine | - |
? | |
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | Mycobacterium tuberculosis ATCC 25618 | - |
diphosphate + UDP-N-acetyl-alpha-D-glucosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WMN3 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WMN3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL12(DE3) by cobalt affinity chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the recombinant enzyme binds to human interleukin-8, interaction analysis with human neutrophils, overview | Mycobacterium tuberculosis | ? | - |
? | |
additional information | the recombinant enzyme binds to human interleukin-8, interaction analysis with human neutrophils, overview | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
additional information | the recombinant enzyme binds to human interleukin-8, interaction analysis with human neutrophils, overview | Mycobacterium tuberculosis ATCC 25618 | ? | - |
? | |
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
Mycobacterium tuberculosis | diphosphate + UDP-N-acetyl-alpha-D-glucosamine | - |
? | |
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
Mycobacterium tuberculosis H37Rv | diphosphate + UDP-N-acetyl-alpha-D-glucosamine | - |
? | |
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
Mycobacterium tuberculosis ATCC 25618 | diphosphate + UDP-N-acetyl-alpha-D-glucosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GlmU | - |
Mycobacterium tuberculosis |
N-acetylglucosamine-1-phosphate uridyltransferase | - |
Mycobacterium tuberculosis |
Rv1018c | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is one of the pathogen proteins that bind to human interleukin-8, IL-8. The binding interaction of mycobacterial proteins AtsG, GlmU and SahH with human IL-8 may indicate that these proteins participate in the modulation of the early events of infection with tubercle bacilli and could affect pathogen attachment to target cells. Interleukin-8 belongs to the family of CXC chemokines and functions as a chemoattractant and activator of different subsets of leukocytes | Mycobacterium tuberculosis |