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Literature summary for 2.7.7.23 extracted from

  • Dziadek, B.; Brzostek, A.; Grzybowski, M.; Fol, M.; Krupa, A.; Kryczka, J.; Plocinski, P.; Kurdowska, A.; Dziadek, J.
    Mycobacterium tuberculosis AtsG (Rv0296c), GlmU (Rv1018c) and SahH (Rv3248c) proteins function as the human IL-8-binding effectors and contribute to pathogen entry into human neutrophils (2016), PLoS ONE, 11, e0148030.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene glmU, recombinant expression of His-tagged enzyme in Escherichia coli strain BL12(DE3), overexpression of GlmU in Mycobacterium tuberculosis Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
additional information construction of Mycobacterium tuberculosis mutant strains overproducing GlmU allows determination of the contribution of the protein to mycobacterial entry into human neutrophils Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate Mycobacterium tuberculosis
-
diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
?
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate Mycobacterium tuberculosis H37Rv
-
diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
?
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate Mycobacterium tuberculosis ATCC 25618
-
diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WMN3
-
-
Mycobacterium tuberculosis H37Rv P9WMN3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL12(DE3) by cobalt affinity chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the recombinant enzyme binds to human interleukin-8, interaction analysis with human neutrophils, overview Mycobacterium tuberculosis ?
-
?
additional information the recombinant enzyme binds to human interleukin-8, interaction analysis with human neutrophils, overview Mycobacterium tuberculosis H37Rv ?
-
?
additional information the recombinant enzyme binds to human interleukin-8, interaction analysis with human neutrophils, overview Mycobacterium tuberculosis ATCC 25618 ?
-
?
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
Mycobacterium tuberculosis diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
?
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
Mycobacterium tuberculosis H37Rv diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
?
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
Mycobacterium tuberculosis ATCC 25618 diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
?

Synonyms

Synonyms Comment Organism
GlmU
-
Mycobacterium tuberculosis
N-acetylglucosamine-1-phosphate uridyltransferase
-
Mycobacterium tuberculosis
Rv1018c
-
Mycobacterium tuberculosis

General Information

General Information Comment Organism
physiological function the enzyme is one of the pathogen proteins that bind to human interleukin-8, IL-8. The binding interaction of mycobacterial proteins AtsG, GlmU and SahH with human IL-8 may indicate that these proteins participate in the modulation of the early events of infection with tubercle bacilli and could affect pathogen attachment to target cells. Interleukin-8 belongs to the family of CXC chemokines and functions as a chemoattractant and activator of different subsets of leukocytes Mycobacterium tuberculosis