Literature summary for 2.7.7.23 extracted from

Zhou, Y.; Xin, Y.; Sha, S.; Ma, Y.
Kinetic properties of Mycobacterium tuberculosis bifunctional GlmU (2011), Arch. Microbiol., 193, 751-757.

Search for more literature for 2.7.7.23

Application

Application	Comment	Organism
analysis	quick and sensitive enzyme detection assay based on the diphosphatase-coupled colorimetric method with malachite green, and assay for glucosamine-1-phosphate N-acetyltransferase activity of the bifunctional enzyme, both applicable in microtitter plates	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.024	-	UTP	pH 7.5, 37°C	Mycobacterium tuberculosis
0.044	-	N-acetyl-D-glucosamine 1-phosphate	pH 7.5, 37°C	Mycobacterium tuberculosis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	absolutely required, optimal concentration 20 mM	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WMN3	bifunctional UDP-N-acetylglucosamine diphosphorylase, EC 2.7.7.23, and glucosamine-1-phosphate N-acetyltransferase, EC 2.3.1.157	-
Mycobacterium tuberculosis H37Rv	P9WMN3	bifunctional UDP-N-acetylglucosamine diphosphorylase, EC 2.7.7.23, and glucosamine-1-phosphate N-acetyltransferase, EC 2.3.1.157	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-acetyl-D-glucosamine 1-phosphate + UTP	-	Mycobacterium tuberculosis	UDP-N-acetyl-D-glucosamine + diphosphate	-	?
N-acetyl-D-glucosamine 1-phosphate + UTP	-	Mycobacterium tuberculosis H37Rv	UDP-N-acetyl-D-glucosamine + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
GlmU	-	Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.04	-	UTP	pH 7.5, 37°C	Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)