General Stability | Organism |
---|---|
labile toward fractionation with ammonium sulfate, inactivation can not be reversed by dialysis | Bos taurus |
more stable toward ammonium sulfate then calf liver enzyme | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fluoride | inhibits Mg2+ but not Mn2+ activated enzyme | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for maximum activity | Staphylococcus aureus | |
Mg2+ | required for maximum activity | Bos taurus | |
Mn2+ | can partially replace Mg2+ | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + UDP-N-acetyl-D-glucosamine | Staphylococcus aureus | amino sugar metabolism | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | Bos taurus | amino sugar metabolism | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r |
Organic Solvent | Comment | Organism |
---|---|---|
organic solvents | labile to fractionation with | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Staphylococcus aureus |
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
330 | - |
purified enzyme, pH 7.4, 37°C | Bos taurus |
2390 | - |
purified enzyme, pH 7.4, 37°C | Staphylococcus aureus |
Storage Stability | Organism |
---|---|
-20°C, acetone precipitated enzyme, 3 months, no loss of activity | Bos taurus |
-20°C, crude extract, 5-10% loss of activity per day of storage | Bos taurus |
-20°C, purified enzyme or crude extract, 2 months, 10% loss of activity | Staphylococcus aureus |
-20°C, purified enzyme, 10% loss of activity per week of storage | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + UDP-glucose | 30% of the activity with UDP-N-acetylglucosamine | Bos taurus | UTP + D-glucose 1-phosphate | - |
? | |
diphosphate + UDP-N-acetyl-D-galactosamine | 2.8% of the activity with UDP-N-acetylglucosamine | Staphylococcus aureus | UTP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
diphosphate + UDP-N-acetyl-D-glucosamine | - |
Staphylococcus aureus | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | - |
Bos taurus | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | amino sugar metabolism | Staphylococcus aureus | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | amino sugar metabolism | Bos taurus | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Staphylococcus aureus |
7 | - |
- |
Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | - |
Staphylococcus aureus |