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Literature summary for 2.7.7.23 extracted from
- Purification of uridine diphosphoacetylglucosamine pyrophosphorylase from sheep brain (1961), Biochim. Biophys. Acta, 50, 129-134.
General Stability
| General Stability | Organism |
|---|---|
| stable towards ammonium sulfate, unstable in prolonged dialysis | Ovis aries |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | slight inhibition above 8 mM | Ovis aries |  |
| EDTA | not reversible by addition of Mg2+ | Ovis aries | |
| p-chloromercuribenzoate | completely reversible with cysteine | Ovis aries | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.36 | - |
UDP-N-acetyl-D-glucosamine | pH 7.4, 37°C | Ovis aries | |
| 0.79 | - |
diphosphate | pH 7.4, 37°C | Ovis aries | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can partially replace Mg2+ | Ovis aries | |
| Mg2+ | required for maximum activity | Ovis aries | |
| Mn2+ | can partially replace Mg2+ | Ovis aries | |
| Ni2+ | inhibitory | Ovis aries | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + UDP-N-acetyl-D-glucosamine | Ovis aries | amino sugar metabolism | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ovis aries |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.17 | - |
purified enzyme, pH 7.4, 37°C | Ovis aries |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 1 month, 40% loss of activity | Ovis aries |
| 0°C, 2 weeks, 50% loss of activity | Ovis aries |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + UDP-N-acetyl-D-glucosamine | - |
Ovis aries | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | amino sugar metabolism | Ovis aries | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Ovis aries |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | - |
Ovis aries |
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Release 2023.1 (January 2023)
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