Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | product inhibition | Rattus norvegicus | |
FAD | strong product inhibitor, 50% inhibition at 0.006 mM, competitive inhibition against ATP, mixed inhibition against FMN | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 30% of the activity with Mg2+ | Rattus norvegicus | |
Mg2+ | - |
Rattus norvegicus | |
Mg2+ | - |
Bos taurus | |
Mn2+ | about 15% of the activity with Mg2+ | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
2 * 53000, SDS-PAGE | Rattus norvegicus |
97000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + FMN | Bos taurus | - |
diphosphate + FAD | - |
r | |
ATP + FMN | Corynebacterium ammoniagenes | - |
diphosphate + FAD | - |
r | |
ATP + FMN | Rattus norvegicus | biosynthesis of FAD is most likely regulated by this coenzyme as a product at the stage of FAD synthetase reaction | diphosphate + FAD | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
bovine | - |
Corynebacterium ammoniagenes | - |
ATCC6872 and KY13315, previously Brevibacterium ammoniagenes | - |
Rattus norvegicus | - |
rat | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
- |
Bos taurus |
- |
Corynebacterium ammoniagenes |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0062 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
5°C, if dithiothreitol is removed from the purified enzyme solution enzyme denatures within 12 h | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + FMN | - |
Rattus norvegicus | diphosphate + FAD | - |
r | |
ATP + FMN | - |
Bos taurus | diphosphate + FAD | - |
r | |
ATP + FMN | - |
Corynebacterium ammoniagenes | diphosphate + FAD | - |
r | |
ATP + FMN | biosynthesis of FAD is most likely regulated by this coenzyme as a product at the stage of FAD synthetase reaction | Rattus norvegicus | diphosphate + FAD | - |
? | |
additional information | - |
Rattus norvegicus | ? | - |
? | |
additional information | bifunctional FAD synthetase which shows FMN adenylyltransferase and flavokinase activities, producing FMN ATP:riboflavin 5'-phosphotransferase EC 2.7.1.26 | Corynebacterium ammoniagenes | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 53000, SDS-PAGE | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.1 | - |
- |
Bos taurus |
7.1 | - |
- |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | nucleoside triphosphates other than ATP do not act as substrates or inhibitors | Rattus norvegicus | |
ATP | nucleoside triphosphates other than ATP do not act as substrates or inhibitors | Corynebacterium ammoniagenes |