General Stability | Organism |
---|---|
thiol maintains stability | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 1 mM inhibits activity by 60% | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0094 | - |
FMN | pH 7.5, 37°C | Rattus norvegicus | |
0.053 | - |
ATP | pH 7.5, 37°C | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 30% of the activity with Mg2+ | Rattus norvegicus | |
Mg2+ | - |
Rattus norvegicus | |
Mn2+ | about 15% of the activity with Mg2+ | Rattus norvegicus | |
additional information | Zn2+ cannot replace Mg2+ | Rattus norvegicus | |
additional information | Ba2+, Co2+, Cu2+, Cd2+, Fe2+, Ni2+, Sn2+ and Sr2+ do not show any activity | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
2 * 53000, SDS-PAGE | Rattus norvegicus |
97000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + FMN | Rattus norvegicus | - |
diphosphate + FAD | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
rat | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.133 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, 1 mM dithiothreitol, 20% glycerol can be stored without significant loss of activity for 1 week | Rattus norvegicus |
5°C, if dithiothreitol is removed from the purified enzyme solution enzyme denatures within 12 h | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + FMN | - |
Rattus norvegicus | diphosphate + FAD | - |
ir | |
ATP + FMN | essentially irreversible in the direction of FAD formation | Rattus norvegicus | diphosphate + FAD | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 53000, SDS-PAGE | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
enzyme denatured as temperature increases, completely inactivated above, bovine serum albumin stabilizes up to 45°C | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
- |
Rattus norvegicus |