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Literature summary for 2.7.7.2 extracted from
- Reversible enzymatic synthesis of flavin-adenine dinucleotide (1950), J. Biol. Chem., 182, 795-803.
General Stability
| General Stability | Organism |
|---|---|
| purified enzyme is instable | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | inhibits FAD cleavage completely | Saccharomyces cerevisiae |  |
| NAD+ | inhibits FAD cleavage completely | Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for FAD synthesis, optimal concentration 1.5 mM, inhibition at higher levels | Saccharomyces cerevisiae | |
| additional information | Zn2+ cannot replace Mg2+ | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + FMN | Saccharomyces cerevisiae | - |
diphosphate + FAD | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
baker's yeast, brewers' yeast, beer yeast | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00138 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 3°C, loses 20-30% of its activity in 4 days, 58% in 11 days | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + FMN | - |
Saccharomyces cerevisiae | diphosphate + FAD | - |
r | |
| diphosphate + FAD | - |
Saccharomyces cerevisiae | ATP + FMN | - |
r | |
| additional information | in the reverse reaction diphosphate cannot be replaced by orthophosphate or metaphosphate | Saccharomyces cerevisiae | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.4 | at pH 6.0 10% as active as at pH 7.5, at pH 8.4 64% | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | no FAD synthesis in absence of ATP, ADP cannot replace ATP | Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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