General Stability | Organism |
---|---|
purified enzyme is instable | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | inhibits FAD cleavage completely | Saccharomyces cerevisiae | |
NAD+ | inhibits FAD cleavage completely | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for FAD synthesis, optimal concentration 1.5 mM, inhibition at higher levels | Saccharomyces cerevisiae | |
additional information | Zn2+ cannot replace Mg2+ | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + FMN | Saccharomyces cerevisiae | - |
diphosphate + FAD | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
baker's yeast, brewers' yeast, beer yeast | - |
Purification (Comment) | Organism |
---|---|
partial | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00138 | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
3°C, loses 20-30% of its activity in 4 days, 58% in 11 days | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + FMN | - |
Saccharomyces cerevisiae | diphosphate + FAD | - |
r | |
diphosphate + FAD | - |
Saccharomyces cerevisiae | ATP + FMN | - |
r | |
additional information | in the reverse reaction diphosphate cannot be replaced by orthophosphate or metaphosphate | Saccharomyces cerevisiae | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8.4 | at pH 6.0 10% as active as at pH 7.5, at pH 8.4 64% | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | no FAD synthesis in absence of ATP, ADP cannot replace ATP | Saccharomyces cerevisiae |