Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.19 extracted from

  • Rammelt, C.; Bilen, B.; Zavolan, M.; Keller, W.
    PAPD5, a noncanonical poly(A) polymerase with an unusual RNA-binding motif (2011), RNA, 17, 1737-1746.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.19

Cloned(Commentary)

Cloned (Comment) Organism
expression in HEK-293 cell Homo sapiens

Protein Variants

Protein Variants Comment Organism
K560E mutation in the C-terminal basic motif. About 20% of wild-type activity Homo sapiens
additional information deletion variant of the C-terminal part lacking amino acids 369-551. Whereas the C terminus binds to RNA, the deletion variant shows no shift of the RNA in EMSA experiments Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00092
-
 (A)15 wild-type, pH 7.5, 37°C Homo sapiens
0.00319
-
 (A)15 mutant K560E, pH 7.5, 37°C Homo sapiens
0.00534
-
 (A)15 C-terminal deletion mutant, pH 7.5, 37°C Homo sapiens
0.0469
-
 ATP mutant K560E, pH 7.5, 37°C Homo sapiens
0.0636
-
 ATP wild-type, pH 7.5, 37°C Homo sapiens
0.0643
-
 ATP C-terminal deletion mutant, pH 7.5, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Homo sapiens 5634
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8NDF8
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(A)15 + n ATP in the presence of ATP, the incorporation of several nucleotides into the RNA substrate is observed Homo sapiens (A)15+n + n diphosphate
-
 ?
ATP + 3' untranslated region of mRNA
-
 Homo sapiens diphosphate + ?
-
 ?
ATP + oligo(A)n
-
 Homo sapiens diphosphate + oligo(A)n+1
-
 ?
ATP + oligo(U)n
-
 Homo sapiens diphosphate + ?
-
 ?
ATP + rRNA
-
 Homo sapiens diphosphate + ?
-
 ?
ATP + yeast tRNAiMet in vitro-synthesized yeast tRNAiMet, but not the native tRNA is substrate Homo sapiens diphosphate + ?
-
 ?
additional information isoform PapD5 catalyzes the polyadenylation of different types of RNA substrates in vitro. PAPD5 is active without a protein cofactor. The C terminus of PpaD5 contains a stretch of basic amino acids that is involved in binding the RNA substrate. Incorporation of UTP, GTP, CTP is low and limited to single residues, showing a strong preference of isoform PAPD5 for ATP. No substrates: dNTPs Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
PapD5
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.027
-
 ATP C-terminal deletion mutant, pH 7.5, 37°C Homo sapiens
0.028
-
 ATP mutant K560E, pH 7.5, 37°C Homo sapiens
0.097
-
 ATP wild-type, pH 7.5, 37°C Homo sapiens
0.142
-
 (A)15 mutant K560E, pH 7.5, 37°C Homo sapiens
0.147
-
 (A)15 C-terminal deletion mutant, pH 7.5, 37°C Homo sapiens
0.18
-
 (A)15 wild-type, pH 7.5, 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
27.5
-
 (A)15 C-terminal deletion mutant, pH 7.5, 37°C Homo sapiens
44.4
-
 (A)15 mutant K560E, pH 7.5, 37°C Homo sapiens
195.7
-
 (A)15 wild-type, pH 7.5, 37°C Homo sapiens