Literature summary for 2.7.7.19 extracted from

Just, A.; Butter, F.; Trenkmann, M.; Heitkam, T.; Moerl, M.; Betat, H.
A comparative analysis of two conserved motifs in bacterial poly(A) polymerase and CCA-adding enzyme (2008), Nucleic Acids Res., 36, 5212-5220.

Search for more literature for 2.7.7.19

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) or BL21(DE3)pLys cells	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
D212A	mutant enzyme forms specifically incorporated A-residues with a fidelity comparable to that of the wild type enzyme	Escherichia coli
E211A	mutant enzyme forms specifically incorporated A-residues with a fidelity comparable to that of the wild type enzyme	Escherichia coli
R215A	the mutation leads to a dramatic loss in nucleotide specificity and the formation of poly(N) tails	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0ABF1	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap chelating Sepharose column chromatography	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + RNA	-	Escherichia coli	diphosphate + RNA(A)n	-	?

Synonyms

Synonyms	Comment	Organism
PAP	-	Escherichia coli
poly(A) polymerase	-	Escherichia coli

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Release 2023.1 (January 2023)