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Literature summary for 2.7.7.19 extracted from

  • Balbo, P.B.; Toth, J.; Bohm, A.
    X-ray crystallographic and steady state fluorescence characterization of the protein dynamics of yeast polyadenylate polymerase (2007), J. Mol. Biol., 366, 1401-1415.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.19

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, using 20% (w/v) PEG 8000, 100 mM magnesium acetate, 100 mM imidazole (pH 6.2), 3% ethylene glycol Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0197
-
 2-aminopurine riboside triphosphate
-
 Saccharomyces cerevisiae
0.0359
-
 ATP
-
 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-aminopurine riboside triphosphate + RNA Saccharomyces cerevisiae
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P29468
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-affinity column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminopurine riboside triphosphate + RNA
-
 Saccharomyces cerevisiae ?
-
 ?
ATP + RNA PAP catalyzes the synthesis of poly(A) tails on the 3'-end of pre-mRNA Saccharomyces cerevisiae diphosphate + RNA(A)n
-
 ?

Synonyms

Synonyms Comment Organism
PAP
-
 Saccharomyces cerevisiae
polyadenylate polymerase
-
 Saccharomyces cerevisiae