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Literature summary for 2.7.7.19 extracted from
- Isolation and partial characterization of the poly(A) polymerases from HeLa cells infected with vaccinia virus (1977), J. Biol. Chem., 252, 6939-6947.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | infected cytoplasm of HeLa cells | Homo sapiens | 5737 | - |
| nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | HeLa cells contain one enzyme form that is stimulated by Mn2+ and also by Mg2+, and a second one that is absolutely dependent on the presence of Mg2+ | Homo sapiens |  |
| Mg2+ | Vaccinia virus enzyme is stimulated by Mn2+ and also by Mg2+ | Vaccinia virus | |
| Mn2+ | HeLa cells contain one enzyme form that is stimulated by Mn2+ and also by Mg2+, and a second one that is absolutely dependent on the presence of Mg2+ | Homo sapiens | |
| Mn2+ | Vaccinia virus enzyme is stimulated by Mn2+ and also by Mg2+ | Vaccinia virus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
1 * 37000 + 1 * 57000, SDS-PAGE | Vaccinia virus |
| 50000 | - |
1 * 50000, nuclear Mn2+- and Mg2+-activated enzyme, SDS-PAGE | Homo sapiens |
| 57000 | - |
1 * 37000 + 1 * 57000, SDS-PAGE | Vaccinia virus |
| 58000 | - |
nuclear Mg2+- and Mn2+-stimulated enzyme, glycerol density gradient sedimentation | Homo sapiens |
| 63000 | - |
cytoplasmic Mn2+-dependent enzyme, glycerol density gradient sedimentation | Homo sapiens |
| 70000 | - |
enzyme from infected cytoplasm, glycerol density gradient sedimentation | Vaccinia virus |
| 75000 | - |
1 * 75000, cytoplasmic, Mn2+-dependent enzyme, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
HeLa infected with vaccinia virus | - |
| Homo sapiens | - |
2 forms: 1. nuclear enzyme, stimulated by Mn2+ and Mg2+, 2. cytoplasmic, dependent on Mn2+ | - |
| Vaccinia virus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
- |
Vaccinia virus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + RNA | primer required | Homo sapiens | diphosphate + RNA(A)n | - |
? | |
| ATP + RNA | primer required | Vaccinia virus | diphosphate + RNA(A)n | - |
? | |
| ATP + RNA | human nuclear enzyme and Vaccinia virus enzyme are able to use both RNA and oligo(A) as primer, human cytoplasmic enzyme is able to use RNA but not oligo(A) | Homo sapiens | diphosphate + RNA(A)n | - |
? | |
| ATP + RNA | human nuclear enzyme and Vaccinia virus enzyme are able to use both RNA and oligo(A) as primer, human cytoplasmic enzyme is able to use RNA but not oligo(A) | Vaccinia virus | diphosphate + RNA(A)n | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 37000 + 1 * 57000, SDS-PAGE | Vaccinia virus |
| monomer | 1 * 75000, cytoplasmic, Mn2+-dependent enzyme, SDS-PAGE | Homo sapiens |
| monomer | 1 * 50000, nuclear Mn2+- and Mg2+-activated enzyme, SDS-PAGE | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
vaccinia virus enzyme, human, cytoplasmic Mn2+-dependent enzyme | Homo sapiens |
| 8 | - |
vaccinia virus enzyme, human, cytoplasmic Mn2+-dependent enzyme | Vaccinia virus |
| 8.3 | - |
human nuclear Mn2+- and Mg2+-activated enzyme | Homo sapiens |
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