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Literature summary for 2.7.7.19 extracted from
- Nuclear poly(A) polymerase from rat liver and a hepatoma. Comparison of properties, molecular weights and amino acid compositions (1976), Eur. J. Biochem., 67, 11-21.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-Amanitin | - |
Rattus norvegicus |  |
| ATP | hepatoma enzyme less effective to substrate inhibition than liver enzyme | Rattus norvegicus | |
| Cordycepin 5'-triphosphate | - |
Rattus norvegicus | |
| diphosphate | - |
Rattus norvegicus | |
| K+ | KCl; maximal stimulation at 40 mM, inhibition above 250 mM | Rattus norvegicus | |
| N-ethylmaleimide | - |
Rattus norvegicus | |
| Pancreatic ribonuclease | - |
Rattus norvegicus | |
| Rose bengal | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
ATP | 37°C, pH 8.0 | Rattus norvegicus | |
| 0.15 | - |
ATP | 37°C, pH 8.0 | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Rattus norvegicus | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | maximal stimulation at 40 mM, inhibition above 250 mM | Rattus norvegicus | |
| Mg2+ | 10% of the activity with Mn2+ | Rattus norvegicus | |
| Mn2+ | absolute requirement | Rattus norvegicus | |
| Mn2+ | optimal concentration: 0.25-0.75 mM | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
x * 48000, liver enzyme, SDS-PAGE | Rattus norvegicus |
| 60000 | - |
x * 60000, hepatoma enzyme, SDS-PAGE | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatoma cell | Morris hepatoma tumor cells 3924A | Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + RNA | highly specific for ATP | Rattus norvegicus | diphosphate + RNA(A)n | - |
? | |
| ATP + RNA | primer required | Rattus norvegicus | diphosphate + RNA(A)n | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, hepatoma enzyme, SDS-PAGE | Rattus norvegicus |
| ? | x * 48000, liver enzyme, SDS-PAGE | Rattus norvegicus |
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