Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cordycepin | not | Bos taurus | |
Ionic strength | - |
Bos taurus | |
K+ | 100 mM; KCl | Bos taurus | |
additional information | not inhibitory: actinomycin D; not inhibitory: alpha-amanitin | Bos taurus | |
NH4+ | 10-40 mM; ammonium sulfate | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Bos taurus | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | more active in presence of Mg2+ than Mn2+ | Bos taurus | |
Mn2+ | more active in presence of Mg2+ than Mn2+ | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | 160000 | gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Purification (Comment) | Organism |
---|---|
calf | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
thymus | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNA | highly specific for ATP | Bos taurus | diphosphate + RNA(A)n | - |
? | |
ATP + RNA | primer required | Bos taurus | diphosphate + RNA(A)n | - |
? | |
ATP + RNA | no specificity for the 3'-terminal nucleotides | Bos taurus | diphosphate + RNA(A)n | - |
? | |
ATP + RNA | rather low specificity for primer | Bos taurus | diphosphate + RNA(A)n | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Bos taurus |