Literature summary for 2.7.7.18 extracted from

Rodionova, I.A.; Zuccola, H.J.; Sorci, L.; Aleshin, A.E.; Kazanov, M.D.; Ma, C.T.; Sergienko, E.; Rubin, E.J.; Locher, C.P.; Osterman, A.L.
Mycobacterial nicotinate mononucleotide adenylyltransferase: structure, mechanism, and implications for drug discovery (2015), J. Biol. Chem., 290, 7693-7706.

Application

Application	Comment	Organism
drug development	enzyme NadD is a potentially druggable target suitable for the development of selective inhibitors	Mycobacterium tuberculosis
medicine	the enzyme is a target for developing antimycobacterial therapies	Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment)	Organism
gene nadD, sequence comparisons, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant wild-type and W117A mutant enzymes, sitting drop vapour diffusion method at 20°C, microcrystal seeding using hanging drops, mixing of 10 mg/ml protein in 20 mM Tris, pH 7.8, 200 mM NaCl, and 5% v/v glycerol with well solution containing 1.2-1.5 M MgSO4, 0.1 M MES buffer, pH 6.0-6.5, microseeds are added to the drops 1 h later, macrocrystals growth within 3-5 days., X-ray diffraction structure determination and analysis at 2.4 A resolution, molecular replacement	Mycobacterium tuberculosis

Crystallization (Comment)

Organism

purified recombinant wild-type and W117A mutant enzymes, sitting drop vapour diffusion method at 20°C, microcrystal seeding using hanging drops, mixing of 10 mg/ml protein in 20 mM Tris, pH 7.8, 200 mM NaCl, and 5% v/v glycerol with well solution containing 1.2-1.5 M MgSO4, 0.1 M MES buffer, pH 6.0-6.5, microseeds are added to the drops 1 h later, macrocrystals growth within 3-5 days., X-ray diffraction structure determination and analysis at 2.4 A resolution, molecular replacement

Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
D14A	site-directed mutagenesis, almost inactive mutant	Mycobacterium tuberculosis
H17A	site-directed mutagenesis, almost inactive mutant	Mycobacterium tuberculosis
H20A	site-directed mutagenesis, almost inactive mutant	Mycobacterium tuberculosis
K47A	site-directed mutagenesis, the mutant shows a decrease in Km for ATP and a 6fold increase in Km for nicotinate beta-D-ribonucleotide and a nearly abolished enzyme activity	Mycobacterium tuberculosis
L164A	site-directed mutagenesis, the L164A mutant elutes as both a monomer and a dimer during purification, the mutant shows 40fold reduced activity compared to the wild-type enzyme	Mycobacterium tuberculosis
L164Q	site-directed mutagenesis, the L164Q mutant elutes as both a monomer and a dimer during purification, almost inactive mutant	Mycobacterium tuberculosis
P44A	site-directed mutagenesis, the mutant shows a decrease in Km for ATP	Mycobacterium tuberculosis
Q46A	site-directed mutagenesis	Mycobacterium tuberculosis
T12A	site-directed mutagenesis, the T12A mutant elutes as a dimer and as an aggregated protein during purification, the mutant shows highly reduced activity compared to the wild-type enzyme	Mycobacterium tuberculosis
T86A	site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme	Mycobacterium tuberculosis
W117A	site-directed mutagenesis, the mutant shows a 10fold higher expression level compared to the wild-type enzyme in Escherichia coli, three-dimensional structure determination and analysis, the mutant shows highly reduced activity compared to the wild-type enzyme	Mycobacterium tuberculosis
W117F	site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme	Mycobacterium tuberculosis
W45A	site-directed mutagenesis	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism
2-(2,4-dimethylphenyl)-N-(4-([(5-methyl-3-isoxazolyl)amino]sulfonyl)phenyl)-4-quinolinecarboxamide	MIC80 values of 0.02-0.1 mM and above. The compounds show no inhibition of the human enzyme	Mycobacterium tuberculosis
2-(2,4-dimethylphenyl)-N-[4-(1,3-thiazol-2-ylsulfamoyl)phenyl]quinoline-4-carboxamide	-	Mycobacterium tuberculosis
2-(2,4-dimethylphenyl)-N-[4-[(5-methyl-1,2-oxazol-3-yl)sulfamoyl]phenyl]quinoline-4-carboxamide	-	Mycobacterium tuberculosis
2-(2-chlorophenyl)-N-[4-(1,3-thiazol-2-ylsulfamoyl)phenyl]quinoline-4-carboxamide	-	Mycobacterium tuberculosis
2-(4-bromophenyl)-N-[4-[(4-methylpyrimidin-2-yl)sulfamoyl]phenyl]quinoline-4-carboxamide	-	Mycobacterium tuberculosis
2-(furan-2-yl)-N-[3-(3,4,5,6-tetrahydropyridin-2-ylsulfamoyl)phenyl]quinoline-4-carboxamide	-	Mycobacterium tuberculosis
2-(furan-2-yl)-N-[3-methoxy-4-(morpholin-4-ylsulfonyl)phenyl]quinoline-4-carboxamide	-	Mycobacterium tuberculosis
2-(furan-2-yl)-N-[3-methyl-4-(morpholin-4-ylsulfonyl)phenyl]quinoline-4-carboxamide	-	Mycobacterium tuberculosis
2-(pyridin-4-yl)-N-[4-(1,3-thiazol-2-ylsulfamoyl)phenyl]quinoline-4-carboxamide	-	Mycobacterium tuberculosis
2-phenyl-N-[4-(1,3-thiazol-2-ylsulfamoyl)phenyl]quinoline-4-carboxamide	-	Mycobacterium tuberculosis
3-(1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-N-[4-(1,3-thiazol-2-ylsulfamoyl)phenyl]benzamide	-	Mycobacterium tuberculosis
4-[ethyl(phenyl)sulfamoyl]-N-[5-(2-methoxyphenyl)-1,3,4-oxadiazol-2-yl]benzamide	-	Mycobacterium tuberculosis
additional information	inhibitory profiling of partially active mutants and wild-type enzyme MtNadD using small molecule inhibitors, overview. Substrate inhibition in an ordered bi-reactant system	Mycobacterium tuberculosis
N-[3-(3,4-dihydro-2H-pyrrol-5-ylsulfamoyl)phenyl]-2-(furan-2-yl)quinoline-4-carboxamide	-	Mycobacterium tuberculosis
N-[3-[1-(6-chloro-4-phenylquinazolin-2-yl)-5-(furan-2-yl)-4,5-dihydro-1H-pyrazol-3-yl]phenyl]methanesulfonamide	-	Mycobacterium tuberculosis
N-[4-(3,4-dihydro-2H-pyrrol-5-ylsulfamoyl)phenyl]-2-(furan-2-yl)quinoline-4-carboxamide	-	Mycobacterium tuberculosis
N-[4-(4-iodophenyl)-1,3-thiazol-2-yl]-3-[(phenylsulfonyl)amino]benzamide	-	Mycobacterium tuberculosis
N-[4-(4-methoxyphenyl)-1,3-thiazol-2-yl]-3-[(phenylsulfonyl)amino]benzamide	-	Mycobacterium tuberculosis
N-[4-[(3,4-dimethyl-1,2-oxazol-5-yl)sulfamoyl]-3-methylphenyl]-2-(4-methoxyphenyl)quinoline-4-carboxamide	-	Mycobacterium tuberculosis
N-[4-[(3,4-dimethyl-1,2-oxazol-5-yl)sulfamoyl]-3-methylphenyl]-2-(4-methylphenyl)quinoline-4-carboxamide	-	Mycobacterium tuberculosis
S-(4-bromo-8-chloronaphthalen-1-yl) [4-(1,3-thiazol-2-ylsulfamoyl)phenyl]carbamothioate	-	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
additional information	-	additional information	steady-state kinetic analysis, sequential kinetic mechanism, bisubstrate enzyme kinetics, overview	Mycobacterium tuberculosis
0.006	-	ATP	pH 7.5, 37°C, recombinant mutant K47A	Mycobacterium tuberculosis
0.011	-	ATP	pH 7.5, 37°C, recombinant mutant T86A	Mycobacterium tuberculosis
0.011	-	ATP	pH 7.5, 37°C, recombinant mutant W45A	Mycobacterium tuberculosis
0.017	-	ATP	pH 7.5, 37°C, recombinant mutant P44A	Mycobacterium tuberculosis
0.023	-	ATP	pH 7.5, 37°C, recombinant mutant W117F	Mycobacterium tuberculosis
0.033	-	ATP	pH 7.5, 37°C, recombinant mutant Q46A	Mycobacterium tuberculosis
0.041	-	ATP	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis
0.042	-	ATP	pH 7.5, 37°C, recombinant mutant T12A	Mycobacterium tuberculosis
0.17	-	ATP	pH 7.5, 37°C, recombinant mutant L164A	Mycobacterium tuberculosis
0.19	-	ATP	pH 7.5, 37°C, recombinant mutant L164Q	Mycobacterium tuberculosis
0.33	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant Q46A	Mycobacterium tuberculosis
0.43	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant L164Q	Mycobacterium tuberculosis
0.48	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis
0.52	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant L164A	Mycobacterium tuberculosis
0.53	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant T12A	Mycobacterium tuberculosis
0.84	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant P44A	Mycobacterium tuberculosis
0.98	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant W45A	Mycobacterium tuberculosis
1.13	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant W117F	Mycobacterium tuberculosis
2.67	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant W117A	Mycobacterium tuberculosis
2.7	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant K47A	Mycobacterium tuberculosis
2.8	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant T86A	Mycobacterium tuberculosis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27000	-	2 * 27000, SDS-PAGE	Mycobacterium tuberculosis
54000	-	about, gel filtration	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + nicotinate beta-D-ribonucleotide	Mycobacterium tuberculosis	-	diphosphate + deamido-NAD+	-	?
ATP + nicotinate beta-D-ribonucleotide	Mycobacterium tuberculosis H37Rv	-	diphosphate + deamido-NAD+	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WJJ5	gene nadD	-
Mycobacterium tuberculosis H37Rv	P9WJJ5	gene nadD	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage from wild-type enzyme and W117A mutant enzyme through Ulp1 protease or TEV protease, and anion exchange chromatography and dialysis	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + nicotinate beta-D-ribonucleotide	-	Mycobacterium tuberculosis	diphosphate + deamido-NAD+	-	?
ATP + nicotinate beta-D-ribonucleotide	-	Mycobacterium tuberculosis H37Rv	diphosphate + deamido-NAD+	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 27000, SDS-PAGE	Mycobacterium tuberculosis
More	three-dimensional structure enzyme structure determination and analysis, crystal structure analysis	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
nadD	-	Mycobacterium tuberculosis
nicotinate mononucleotide adenylyltransferase	-	Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.002	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant W117A	Mycobacterium tuberculosis
0.015	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant L164Q	Mycobacterium tuberculosis
0.02	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant K47A	Mycobacterium tuberculosis
0.03	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant W117F	Mycobacterium tuberculosis
0.13	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant L164A	Mycobacterium tuberculosis
0.2	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant T86A	Mycobacterium tuberculosis
0.22	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant T12A	Mycobacterium tuberculosis
0.36	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant P44A	Mycobacterium tuberculosis
0.37	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant W45A	Mycobacterium tuberculosis
4.7	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant Q46A	Mycobacterium tuberculosis
5.5	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Mycobacterium tuberculosis

Cofactor

Cofactor	Comment	Organism	Structure
ATP	binding site structure, overview	Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
additional information	-	additional information	inhibition kinetics, overview	Mycobacterium tuberculosis
0.024	-	2-(2,4-dimethylphenyl)-N-(4-([(5-methyl-3-isoxazolyl)amino]sulfonyl)phenyl)-4-quinolinecarboxamide	pH 7.5, 37°C, recombinant mutant T12A	Mycobacterium tuberculosis
0.03	-	2-(2,4-dimethylphenyl)-N-(4-([(5-methyl-3-isoxazolyl)amino]sulfonyl)phenyl)-4-quinolinecarboxamide	pH 7.5, 37°C, recombinant mutant W117A	Mycobacterium tuberculosis
0.035	-	2-(2,4-dimethylphenyl)-N-(4-([(5-methyl-3-isoxazolyl)amino]sulfonyl)phenyl)-4-quinolinecarboxamide	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis
0.037	-	2-(2,4-dimethylphenyl)-N-(4-([(5-methyl-3-isoxazolyl)amino]sulfonyl)phenyl)-4-quinolinecarboxamide	pH 7.5, 37°C, recombinant mutant P44A	Mycobacterium tuberculosis
0.056	-	2-(2,4-dimethylphenyl)-N-(4-([(5-methyl-3-isoxazolyl)amino]sulfonyl)phenyl)-4-quinolinecarboxamide	pH 7.5, 37°C, recombinant mutant W45A	Mycobacterium tuberculosis
0.094	-	2-(2,4-dimethylphenyl)-N-(4-([(5-methyl-3-isoxazolyl)amino]sulfonyl)phenyl)-4-quinolinecarboxamide	pH 7.5, 37°C, recombinant mutant K47A	Mycobacterium tuberculosis
0.114	-	2-(2,4-dimethylphenyl)-N-(4-([(5-methyl-3-isoxazolyl)amino]sulfonyl)phenyl)-4-quinolinecarboxamide	pH 7.5, 37°C, recombinant mutant T86A	Mycobacterium tuberculosis

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor
0.017	-	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis	2-(furan-2-yl)-N-[3-(3,4,5,6-tetrahydropyridin-2-ylsulfamoyl)phenyl]quinoline-4-carboxamide
0.024	-	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis	3-(1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-N-[4-(1,3-thiazol-2-ylsulfamoyl)phenyl]benzamide
0.024	-	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis	S-(4-bromo-8-chloronaphthalen-1-yl) [4-(1,3-thiazol-2-ylsulfamoyl)phenyl]carbamothioate
0.033	-	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis	2-(2-chlorophenyl)-N-[4-(1,3-thiazol-2-ylsulfamoyl)phenyl]quinoline-4-carboxamide
0.035	-	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis	2-(2,4-dimethylphenyl)-N-[4-[(5-methyl-1,2-oxazol-3-yl)sulfamoyl]phenyl]quinoline-4-carboxamide
0.036	-	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis	2-(2,4-dimethylphenyl)-N-[4-(1,3-thiazol-2-ylsulfamoyl)phenyl]quinoline-4-carboxamide
0.043	-	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis	2-phenyl-N-[4-(1,3-thiazol-2-ylsulfamoyl)phenyl]quinoline-4-carboxamide
0.046	-	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis	N-[4-(3,4-dihydro-2H-pyrrol-5-ylsulfamoyl)phenyl]-2-(furan-2-yl)quinoline-4-carboxamide
0.049	-	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis	N-[3-(3,4-dihydro-2H-pyrrol-5-ylsulfamoyl)phenyl]-2-(furan-2-yl)quinoline-4-carboxamide
0.049	-	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis	2-(4-bromophenyl)-N-[4-[(4-methylpyrimidin-2-yl)sulfamoyl]phenyl]quinoline-4-carboxamide
0.051	-	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis	2-(furan-2-yl)-N-[3-methyl-4-(morpholin-4-ylsulfonyl)phenyl]quinoline-4-carboxamide
0.056	-	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis	2-(pyridin-4-yl)-N-[4-(1,3-thiazol-2-ylsulfamoyl)phenyl]quinoline-4-carboxamide
0.074	-	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis	2-(furan-2-yl)-N-[3-methoxy-4-(morpholin-4-ylsulfonyl)phenyl]quinoline-4-carboxamide
0.096	-	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis	4-[ethyl(phenyl)sulfamoyl]-N-[5-(2-methoxyphenyl)-1,3,4-oxadiazol-2-yl]benzamide
0.116	-	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis	N-[4-(4-methoxyphenyl)-1,3-thiazol-2-yl]-3-[(phenylsulfonyl)amino]benzamide
0.13	-	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis	N-[4-[(3,4-dimethyl-1,2-oxazol-5-yl)sulfamoyl]-3-methylphenyl]-2-(4-methylphenyl)quinoline-4-carboxamide
0.13	-	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis	N-[4-[(3,4-dimethyl-1,2-oxazol-5-yl)sulfamoyl]-3-methylphenyl]-2-(4-methoxyphenyl)quinoline-4-carboxamide
0.13	-	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis	N-[3-[1-(6-chloro-4-phenylquinazolin-2-yl)-5-(furan-2-yl)-4,5-dihydro-1H-pyrazol-3-yl]phenyl]methanesulfonamide
0.18	-	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis	N-[4-(4-iodophenyl)-1,3-thiazol-2-yl]-3-[(phenylsulfonyl)amino]benzamide

General Information

General Information	Comment	Organism
additional information	the enzyme has a unique 310 helix that locks the active site in an over-closed conformation. This renders the enzyme inactive as it is topologically incompatible with substrate binding and catalysis. The enzyme contains the signature motifs GXFXPX(H/T)XXH and SXTXXR mainly implicated in ATP binding. Mechanistic modelling, overview	Mycobacterium tuberculosis
physiological function	nicotinate mononucleotide adenylyltransferase NadD is an essential enzyme in the biosynthesis of the NAD+ cofactor	Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
0.0008	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant W117A	Mycobacterium tuberculosis
0.007	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant K47A	Mycobacterium tuberculosis
0.008	-	ATP	pH 7.5, 37°C, recombinant mutant L164Q	Mycobacterium tuberculosis
0.03	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant W117F	Mycobacterium tuberculosis
0.04	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant L164Q	Mycobacterium tuberculosis
0.07	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant T86A	Mycobacterium tuberculosis
0.08	-	ATP	pH 7.5, 37°C, recombinant mutant L164A	Mycobacterium tuberculosis
0.4	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant P44A	Mycobacterium tuberculosis
0.4	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant T12A	Mycobacterium tuberculosis
0.4	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant W45A	Mycobacterium tuberculosis
1.3	-	ATP	pH 7.5, 37°C, recombinant mutant W117F	Mycobacterium tuberculosis
2.5	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant L164A	Mycobacterium tuberculosis
3	-	ATP	pH 7.5, 37°C, recombinant mutant K47A	Mycobacterium tuberculosis
5	-	ATP	pH 7.5, 37°C, recombinant mutant T12A	Mycobacterium tuberculosis
12	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis
14	-	nicotinate beta-D-ribonucleotide	pH 7.5, 37°C, recombinant mutant Q46A	Mycobacterium tuberculosis
18	-	ATP	pH 7.5, 37°C, recombinant mutant T86A	Mycobacterium tuberculosis
21	-	ATP	pH 7.5, 37°C, recombinant mutant P44A	Mycobacterium tuberculosis
34	-	ATP	pH 7.5, 37°C, recombinant mutant W45A	Mycobacterium tuberculosis
134	-	ATP	pH 7.5, 37°C, recombinant wild-type enzyme	Mycobacterium tuberculosis
142	-	ATP	pH 7.5, 37°C, recombinant mutant Q46A	Mycobacterium tuberculosis