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Literature summary for 2.7.7.15 extracted from

  • Lee, J.; Johnson, J.; Ding, Z.; Paetzel, M.; Cornell, R.B.
    Crystal structure of a mammalian CTP: phosphocholine cytidylyltransferase catalytic domain reveals novel active site residues within a highly conserved nucleotidyltransferase fold (2009), J. Biol. Chem., 284, 33535-33548.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.15

Cloned(Commentary)

Cloned (Comment) Organism
deletion construct 1-236: expressed in T.ni cell using a baculovirus expression system, expressed in Escherichia coli BL21 Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor-diffusion method Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
H168A involved in catalysis Rattus norvegicus
H168A/Y173A involved in catalysis Rattus norvegicus
additional information deletion construct 1-236 (lacks the regulatory domain, displays constitutive activity) Rattus norvegicus
Y173A involved in catalysis Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
 choline phosphate wild-type deletion construct 1-236, 8.8 mM CTP Rattus norvegicus
0.48
-
 choline phosphate Y173A mutant deletion construct 1-236, 8.8 mM CTP Rattus norvegicus
2.5
-
 choline phosphate H168A mutant deletion construct 1-236, 8.8 mM CTP Rattus norvegicus
3.2
-
 choline phosphate H168A/Y173A mutant deletion construct 1-236, 8.8 mM CTP Rattus norvegicus
7.3
-
 CTP wild-type deletion construct 1-236, 1.5 mM choline phosphate Rattus norvegicus
7.7
-
 CTP H168A mutant deletion construct 1-236, 1.5 mM choline phosphate Rattus norvegicus
7.7
-
 CTP Y173A mutant deletion construct 1-236, 1.5 mM choline phosphate Rattus norvegicus
8.8
-
 CTP H168A/Y173A mutant deletion construct 1-236, 1.5 mM choline phosphate Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P19836
-
-

Purification (Commentary)

Purification (Comment) Organism
CM- and Blue-Sepharose chromatography, gel filtartion Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.021
-
 H168A/Y173A mutant deletion construct 1-236, variable CTP concentration, 1.5 mM choline phosphate Rattus norvegicus
0.034
-
 H168A mutant deletion construct 1-236, variable CTP concentration, 1.5 mM choline phosphate Rattus norvegicus
0.055
-
 H168A/Y173A mutant deletion construct 1-236, variable choline phosphate concentration, 8.8 mM CTP Rattus norvegicus
0.069
-
 H168A mutant deletion construct 1-236, variable choline phosphate concentration, 8.8 mM CTP Rattus norvegicus
0.116
-
 Y173A mutant deletion construct 1-236, variable choline phosphate concentration, 8.8 mM CTP Rattus norvegicus
0.167
-
 Y173A mutant deletion construct 1-236, variable CTP concentration, 1.5 mM choline phosphate Rattus norvegicus
1.724
-
 wild-type deletion construct 1-236, variable choline phosphate concentration, 8.8 mM CTP Rattus norvegicus
3.399
-
 wild-type deletion construct 1-236, variable CTP concentration, 1.5 mM choline phosphate Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + choline phosphate
-
 Rattus norvegicus diphosphate + CDP-choline
-
 ?

Subunits

Subunits Comment Organism
homodimer deletion construct 1-236, crystall structure analysis Rattus norvegicus

Synonyms

Synonyms Comment Organism
CTP:phosphocholine cytidylyltransferase
-
 Rattus norvegicus