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Literature summary for 2.7.7.15 extracted from

  • Tilley, D.M.; Evans, C.R.; Larson, T.M.; Edwards, K.A.; Friesen, J.A.
    Identification and characterization of the nuclear isoform of Drosophila melanogaster CTP:phosphocholine cytidylyltransferase (2008), Biochemistry, 47, 11838-11846.
    View publication on PubMed
Search for more literature for 2.7.7.15

Activating Compound

Activating Compound Comment Organism Structure
diphosphatidylglycerol
-
 Drosophila melanogaster
additional information phosphatidylethanolamine and phosphatidic acid do not have a significant effect on CCT1 activity Drosophila melanogaster
oleate
-
 Drosophila melanogaster
phosphatidylcholine 6fold enhancement of activity at 0.025 mM Drosophila melanogaster
phosphatidylglycerol
-
 Drosophila melanogaster
phosphatidylinositol
-
 Drosophila melanogaster
phosphatidylserine
-
 Drosophila melanogaster

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 cells and Schneider 2 cells Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.63
-
 choline phosphate isozyme CCT2 Drosophila melanogaster
0.81
-
 CTP isozyme CCT2 Drosophila melanogaster
1.21
-
 CTP isozyme CCT1 Drosophila melanogaster
2.29
-
 choline phosphate isozyme CCT1 Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm isozyme CCT2 Drosophila melanogaster 5737
-
nucleus isozyme CCT1 Drosophila melanogaster 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+ yields approximately 9% of the activity seen with Mg2+, the cation preference of CCT1 is Mg2+ > Mn2+/Co2+ > Ca2+/Ni2+ > Zn2+ Drosophila melanogaster
Co2+ Co2+ results in significant activity at a concentration of 25 mM, with 41% of the activity seen with 25 mM Mg2+, the cation preference of CCT1 is Mg2+ > Mn2+/Co2+ > Ca2+/Ni2+ > Zn2+ Drosophila melanogaster
Mg2+ the cation preference of CCT1 is Mg2+ > Mn2+/Co2+ > Ca2+/Ni2+ > Zn2+ Drosophila melanogaster
Mn2+ Mn2+ results in significant activity at a concentration of 25 mM, with 51% of the activity seen with 25 mM Mg2+, the cation preference of CCT1 is Mg2+ > Mn2+/Co2+ > Ca2+/Ni2+ > Zn2+ Drosophila melanogaster
Ni2+ Ni2+ yields approximately 9% of the activity seen with Mg2+, the cation preference of CCT1 is Mg2+ > Mn2+/Co2+ > Ca2+/Ni2+ > Zn2+ Drosophila melanogaster
Zn2+ Zn2+ results in catalytic activity of only 1% when compared to the activity in the presence of Mg2+, the cation preference of CCT1 is Mg2+ > Mn2+/Co2+ > Ca2+/Ni2+ > Zn2+ Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q7K4C7
-
-
Drosophila melanogaster Q9W0D9
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon metal affinity column chromatography Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + choline phosphate
-
 Drosophila melanogaster diphosphate + CDP-choline
-
 ?

Synonyms

Synonyms Comment Organism
CCT1 isozyme Drosophila melanogaster
CCT2 isozyme Drosophila melanogaster
CTP:phosphocholine cytidylyltransferase
-
 Drosophila melanogaster