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Literature summary for 2.7.7.15 extracted from

  • Friesen, J.A.; Seo Park, Y.; Kent, C.
    Purification and kinetic characterization of CTP:phosphocholine cytidylyltransferase from Saccharomyces cerevisiae (2001), Protein Expr. Purif., 21, 141-148.
    View publication on PubMed
Search for more literature for 2.7.7.15

Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
Y192H mutant can not be expressed in Escherichia coli Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 phosphocholine presence of lipid Saccharomyces cerevisiae
0.81
-
 CTP absence of lipid Saccharomyces cerevisiae
1.18
-
 phosphocholine absence of lipid Saccharomyces cerevisiae
1.42
-
 CTP presence of lipid Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49400
-
 calculated from amino acid sequence Saccharomyces cerevisiae
60000
-
 SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
196
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + choline phosphate
-
 Saccharomyces cerevisiae diphosphate + CDPcholine
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.51
-
 phosphocholine absence of lipid Saccharomyces cerevisiae
31.3
-
 phosphocholine presence of lipid Saccharomyces cerevisiae