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Literature summary for 2.7.7.15 extracted from
- Enzymic and cellular characterization of a catalytic fragment of CTP:phosphocholine cytidylyltransferase a (1999), J. Biol. Chem., 274, 13384-13389.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| deletion mutant which is truncated after residue 236, called CCTalpha236 | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | deletion mutant which is truncated after residue 236, called CCTalpha236, independent of lipids, 50fold increase in activity | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.65 | - |
phosphocholine | wild-type, presence of lipid | Rattus norvegicus |  |
| 0.9 | - |
phosphocholine | CCTalpha236 mutant, absence of lipid | Rattus norvegicus | |
| 0.97 | - |
phosphocholine | CCTalpha236 mutant, presence of lipid | Rattus norvegicus | |
| 1.05 | - |
phosphocholine | wild-type, absence of lipid | Rattus norvegicus | |
| 1.29 | - |
CTP | wild-type, presence of lipid | Rattus norvegicus | |
| 1.69 | - |
CTP | wild-type, absence of lipid | Rattus norvegicus | |
| 3.15 | - |
CTP | CCTalpha236 mutant, absence of lipid | Rattus norvegicus | |
| 4.47 | - |
CTP | CCTalpha236 mutant, presence of lipid | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50300 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + choline phosphate | Rattus norvegicus | membrane binding segment has important role in cellular lipid metabolism | diphosphate + CDPcholine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| deletion mutant which is truncated after residue 236, called CCTalpha236 | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + choline phosphate | - |
Rattus norvegicus | diphosphate + CDPcholine | - |
? | |
| CTP + choline phosphate | membrane binding segment has important role in cellular lipid metabolism | Rattus norvegicus | diphosphate + CDPcholine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | homodimer, CCTalpha236 | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
phosphocholine | wild-type, absence of lipid | Rattus norvegicus | |
| 11.1 | - |
phosphocholine | CCTalpha236 mutant, presence of lipid | Rattus norvegicus | |
| 12.6 | - |
phosphocholine | CCTalpha236 mutant, absence of lipid | Rattus norvegicus | |
| 17.7 | - |
phosphocholine | wild-type, presence of lipid | Rattus norvegicus | |
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