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Literature summary for 2.7.7.14 extracted from
- Ethanolamine-phosphate cytidylyltransferase (1992), Methods Enzymol., 209, 258-263.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | addition of lipids does not stimulate the activity of cytosolic enzyme | Rattus norvegicus | |
| Reducing agent | activity of purified enzyme is dependent on the presence of DTT | Rattus norvegicus |
General Stability
| General Stability | Organism |
|---|---|
| purified enzyme can be stabilized by the addition of 10% v/v glycerol or 2% bovine serum albumin | Rattus norvegicus |
| quite stable towards freezing and thawing | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.053 | - |
CTP | - |
Rattus norvegicus |  |
| 0.065 | - |
Ethanolamine phosphate | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | predominantly | Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | 120000 | gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + ethanolamine phosphate | Rattus norvegicus | catalyzes a central step in phosphatidylethanolamine synthesis | diphosphate + CDP-ethanolamine | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 1162fold to homogeneity | Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| CTP + ethanolamine phosphate = diphosphate + CDP-ethanolamine | ordered reaction mechanism | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.38 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least 4 weeks without significant loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + ethanolamine phosphate | - |
Rattus norvegicus | diphosphate + CDP-ethanolamine | - |
r | |
| CTP + ethanolamine phosphate | catalyzes a central step in phosphatidylethanolamine synthesis | Rattus norvegicus | diphosphate + CDP-ethanolamine | - |
r | |
| dCTP + ethanolamine phosphate | - |
Rattus norvegicus | diphosphate + dCDP-ethanolamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 49000-50000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
one sharp optimum at pH 7.8 and one with a lower maximal activity around pH 6.0 | Rattus norvegicus |
| 7.8 | - |
one sharp optimum at pH 7.8 and one with a lower maximal activity around pH 6.0 | Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
limited stability below | Rattus norvegicus |
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