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Literature summary for 2.7.7.14 extracted from
- Ethanolaminephosphate cytidylyltransferase. Purification and characterization of the enzyme from rat liver (1975), J. Biol. Chem., 250, 8585-8590.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Reducing agent | like DTT required | Rattus norvegicus | |
| Reducing agent | cysteine or 2-mercaptoethanol are ineffective | Rattus norvegicus |
General Stability
| General Stability | Organism |
|---|---|
| bovine serum albumin, 2% w/v, 1 mM CTP and 20 mM Mg2+, or 10% v/v glycerol stabilizes | Rattus norvegicus |
| enzyme in crude postmicrosomal supernatant is quite stable towards freezing and thawing, but the highly purified enzyme loses 85-90% of its activity when frozen and thawed twice | Rattus norvegicus |
| omission of DTT from buffers used in the later steps of purification results in severe loss of activity | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CDP-ethanolamine | - |
Rattus norvegicus |  |
| CTP | at concentrations exceeding that of Mg2+ | Rattus norvegicus | |
| diphosphate | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.053 | - |
CTP | pH 7.8, 37°C | Rattus norvegicus | |
| 0.065 | - |
Ethanolamine phosphate | pH 7.8, 37°C | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | postmicrosomal supernatant | Rattus norvegicus | 5829 | - |
| additional information | - |
Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation, 10% of the activity with Mg2+ | Rattus norvegicus | |
| Co2+ | activation, 10% of the activity with Mg2+ | Rattus norvegicus | |
| Mg2+ | absolute requirement for a divalent cation | Rattus norvegicus | |
| Mg2+ | 5-10 mM most efficient | Rattus norvegicus | |
| Mn2+ | activation, optimal concentration: 2-4 mM, 50% of the activity with Mg2+ | Rattus norvegicus | |
| additional information | no activation by Ba2+, Zn2+, Cd2+, Ni2+ | Rattus norvegicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | 120000 | gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + ethanolamine phosphate | Rattus norvegicus | catalyzes a central step in phosphatidylethanolamine synthesis | diphosphate + CDP-ethanolamine | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male | - |
| Rattus norvegicus | - |
Sprague-Dawley rats | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| CTP + ethanolamine phosphate = diphosphate + CDP-ethanolamine | ordered reaction mechanism | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.13 | - |
purified enzyme | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, pH 7.5-9.0, in presence of DTT, stable for weeks | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + ethanolamine phosphate | - |
Rattus norvegicus | diphosphate + CDP-ethanolamine | - |
r | |
| CTP + ethanolamine phosphate | catalyzes a central step in phosphatidylethanolamine synthesis | Rattus norvegicus | diphosphate + CDP-ethanolamine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 49000-50000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
one sharp optimum at pH 7.8 and one with a lower maximal activity around pH 6.0 | Rattus norvegicus |
| 7.8 | - |
one sharp optimum at pH 7.8 and one with a lower maximal activity around pH 6.0 | Rattus norvegicus |
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Release 2023.1 (January 2023)
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