Literature summary for 2.7.7.13 extracted from

Zmuda, F.; Shepherd, S.; Ferguson, M.; Gray, D.; Torrie, L.; De Rycker, M.
Trypanosoma cruzi phosphomannomutase and guanosine diphosphate-mannose pyrophosphorylase ligandability assessment (2019), Antimicrob. Agents Chemother., 63, e01082-19 .

Search for more literature for 2.7.7.13

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Trypanosoma cruzi

Inhibitors

Inhibitors	Comment	Organism	Structure
2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)-3-methylbutanoic acid	-	Trypanosoma cruzi
3-(azetidin-1-yl)-N-(imidazo[1,2-a]pyridin-3-ylmethyl)propan-1-amine	-	Trypanosoma cruzi
4-[3-(3-aminopyrrolidin-1-yl)propoxy]benzoic acid	-	Trypanosoma cruzi
5-(2-amino-1,3-thiazol-4-yl)-2-hydroxybenzyl acetate	-	Trypanosoma cruzi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0133	-	alpha-D-mannose 1-phosphate	at pH 7.5 and 25Â°C	Trypanosoma cruzi
0.0407	-	GTP	at pH 7.5 and 25Â°C	Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GTP + alpha-D-mannose 1-phosphate	Trypanosoma cruzi	-	diphosphate + GDP-mannose	-	r
GTP + alpha-D-mannose 1-phosphate	Trypanosoma cruzi CL Brener	-	diphosphate + GDP-mannose	-	r

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma cruzi	Q4CMK4	-	-
Trypanosoma cruzi CL Brener	Q4CMK4	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap Ni affinity column chromatography and Superdex 200 gel filtration	Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + alpha-D-mannose 1-phosphate	-	Trypanosoma cruzi	diphosphate + GDP-mannose	-	r
GTP + alpha-D-mannose 1-phosphate	-	Trypanosoma cruzi CL Brener	diphosphate + GDP-mannose	-	r

Subunits

Subunits	Comment	Organism
hexamer	-	Trypanosoma cruzi

Synonyms

Synonyms	Comment	Organism
GDP-mannose pyrophosphorylase	-	Trypanosoma cruzi
GDP-MP	-	Trypanosoma cruzi
guanosine diphosphate-mannose pyrophosphorylase	-	Trypanosoma cruzi

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Release 2023.1 (January 2023)