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Literature summary for 2.7.7.13 extracted from
- GDP-Mannose pyrophosphorylase is essential in the bloodstream form of Trypanosoma brucei (2010), Biochem. J., 425, 603-614.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His tagged version expressed in Escherichia coli BL21 | Trypanosoma brucei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GDP-6-deoyxmannose | - |
Trypanosoma brucei |  |
| GDP-mannose | feedback inhibition | Trypanosoma brucei | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
D-mannose 1-phosphate | - |
Trypanosoma brucei | |
| 0.067 | - |
GTP | - |
Trypanosoma brucei | |
| 0.29 | - |
ATP | - |
Trypanosoma brucei | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | in bloodstream-form | Trypanosoma brucei | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolute requirement for divalent cations, partially replaced by Mn2+ and Cu2+ | Trypanosoma brucei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
2 * 42000, SDS-PAGE, 2 * 41769.10, ESI-MS (41769.32 theoretical mass) | Trypanosoma brucei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma brucei | B9WNA1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| batch absorption to nickel-agarose | Trypanosoma brucei |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.34 | - |
- |
Trypanosoma brucei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-mannose 1-phosphate | - |
Trypanosoma brucei | diphosphate + ADP-mannose | - |
? | |
| GTP + D-mannose 1-phosphate | - |
Trypanosoma brucei | diphosphate + GDP-mannose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 42000, SDS-PAGE, 2 * 41769.10, ESI-MS (41769.32 theoretical mass) | Trypanosoma brucei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| guanidine diphosphomannose pyrophosphorylase | - |
Trypanosoma brucei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
neutral pH optimum | Trypanosoma brucei |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00125 | - |
GDP-mannose | competitive inhibitor with respect to GTP | Trypanosoma brucei | |
| 0.002 | - |
GDP-mannose | mixed-type inhibition with respect to mannose 1-phosphate | Trypanosoma brucei | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| additional information | - |
IC50 values for ATP and CTP above 0.625 mM | Trypanosoma brucei | additional information | |
| 0.008 | - |
feedback inhibition | Trypanosoma brucei | GDP-mannose | |
| 0.013 | - |
- |
Trypanosoma brucei | GDP-6-deoxymannose | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | essential in bloodstream-form | Trypanosoma brucei |
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Release 2023.1 (January 2023)
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