Literature summary for 2.7.7.13 extracted from

Asencion Diez, M.D.; Demonte, A.; Giacomelli, J.; Garay, S.; Rodrigues, D.; Hofmann, B.; Hecht, H.J.; Guerrero, S.A.; Iglesias, A.A.
Functional characterization of GDP-mannose pyrophosphorylase from Leptospira interrogans serovar Copenhageni (2010), Arch. Microbiol., 192, 103-114.

Search for more literature for 2.7.7.13

Activating Compound

Activating Compound	Comment	Organism	Structure
Mg2+	-	Leptospira interrogans serovar Copenhageni

Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged version expressed in Escherichia coli BL21(DE3)	Leptospira interrogans serovar Copenhageni

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.063	-	alpha-D-mannose 1-phosphate	GDP-mannose synthesis	Leptospira interrogans serovar Copenhageni
0.089	-	diphosphate	pyrophosphorolysis	Leptospira interrogans serovar Copenhageni
0.202	-	GDP-mannose	pyrophosphorolysis	Leptospira interrogans serovar Copenhageni
0.236	-	GTP	GDP-mannose synthesis	Leptospira interrogans serovar Copenhageni
0.466	-	Mg2+	GDP-mannose synthesis	Leptospira interrogans serovar Copenhageni
0.466	-	Mg2+	pyrophosphorolysis	Leptospira interrogans serovar Copenhageni

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	2 * 45000, His-tagged version, gel filtration, SDS-PAGE	Leptospira interrogans serovar Copenhageni

Organism

Organism	UniProt	Comment	Textmining
Leptospira interrogans serovar Copenhageni	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
affinity chromatography using Talon-Co2+ resin, gel filtration	Leptospira interrogans serovar Copenhageni

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.8	-	-	Leptospira interrogans serovar Copenhageni

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + alpha-D-mannose 1-phosphate	-	Leptospira interrogans serovar Copenhageni	diphosphate + GDP-mannose	-	?
GTP + alpha-D-mannose 1-phosphate	60% activity with ITP, 2% activity with UTP, 1% activity with glucose-1-phosphate, and 2% activity with ATP and glucose-1-phosphate	Leptospira interrogans serovar Copenhageni	diphosphate + GDP-alpha-D-mannose	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 45000, His-tagged version, gel filtration, SDS-PAGE	Leptospira interrogans serovar Copenhageni

Synonyms

Synonyms	Comment	Organism
GDP-mannose pyrophosphorylase	-	Leptospira interrogans serovar Copenhageni
mannose-1-phosphate guanylyltransferase	-	Leptospira interrogans serovar Copenhageni

pI Value

Organism	Comment	pI Value Maximum	pI Value
Leptospira interrogans serovar Copenhageni	calculated	-	9.35

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Release 2023.1 (January 2023)