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Literature summary for 2.7.7.13 extracted from

  • Sousa, S.A.; Moreira, L.M.; Wopperer, J.; Eberl, L.; Sa-Correia, I.; Leitao, J.H.
    The Burkholderia cepacia bceA gene encodes a protein with phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities (2007), Biochem. Biophys. Res. Commun., 353, 200-206.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Burkholderia cepacia
gene bceA, the gene is part of the Burkholderia cepacia IST408 exopolysaccharide biosynthetic cluster, physical organization, unrooted phylogenetic tree, DNA and amino acid sequence determination, analysis, and comparisons, overview. Expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Burkholderia cepacia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.9
-
diphosphate in the presence of 10 mM Mn2+ Burkholderia cepacia
2.9
-
GDP-mannose pH 7.6, recombinant enzyme, presence of 10 mM Mn2+ Burkholderia cepacia

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ strongly dependent Burkholderia cepacia
Ca2+ GMP activity is strongly dependent on the presence of Ca2+ or Mn2+ Burkholderia cepacia
Mn2+ strongly dependent Burkholderia cepacia
Mn2+ GMP activity is strongly dependent on the presence of Ca2+ or Mn2+ Burkholderia cepacia
additional information no activation and activity by Ni2+, Mg2+, and Zn2+ Burkholderia cepacia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
SDS-PAGE Burkholderia cepacia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + GDP-mannose Burkholderia cepacia
-
GTP + alpha-D-mannose 1-phosphate
-
?
diphosphate + GDP-mannose Burkholderia cepacia IST408
-
GTP + alpha-D-mannose 1-phosphate
-
?
additional information Burkholderia cepacia the enzyme of Burkholderia cepacia, encoded by gene bceA, is bifunctional exhibiting phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities ?
-
?
additional information Burkholderia cepacia IST408 the enzyme of Burkholderia cepacia, encoded by gene bceA, is bifunctional exhibiting phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities ?
-
?

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
-
-
Burkholderia cepacia Q06XM8 gene bceA
-
Burkholderia cepacia IST408
-
-
-
Burkholderia cepacia IST408 Q06XM8 gene bceA
-

Purification (Commentary)

Purification (Comment) Organism
Hi-Trap column chromatography Burkholderia cepacia
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + GDP-mannose
-
Burkholderia cepacia GTP + alpha-D-mannose 1-phosphate
-
?
diphosphate + GDP-mannose
-
Burkholderia cepacia IST408 GTP + alpha-D-mannose 1-phosphate
-
?
GTP + alpha-D-mannose 1-phosphate
-
Burkholderia cepacia diphosphate + GDP-alpha-D-mannose
-
?
GTP + alpha-D-mannose 1-phosphate
-
Burkholderia cepacia IST408 diphosphate + GDP-alpha-D-mannose
-
?
additional information the enzyme of Burkholderia cepacia, encoded by gene bceA, is bifunctional exhibiting phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities Burkholderia cepacia ?
-
?
additional information the enzyme of Burkholderia cepacia, encoded by gene bceA, is bifunctional exhibiting phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities Burkholderia cepacia IST408 ?
-
?

Synonyms

Synonyms Comment Organism
BceA bifunctional protein with phosphomannose isomerase and GDP-mannose pyrophosphorylase activities Burkholderia cepacia
GDP-D-mannose pyrophosphorylase
-
Burkholderia cepacia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.85
-
diphosphate in the presence of 10 mM Mn2+ Burkholderia cepacia
195.9
-
diphosphate in the presence of 10 mM Mn2+ Burkholderia cepacia
195.9
-
GDP-mannose pH 7.6, recombinant enzyme, presence of 10 mM Mn2+ Burkholderia cepacia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Burkholderia cepacia

pI Value

Organism Comment pI Value Maximum pI Value
Burkholderia cepacia calculated from amino acid sequence
-
6.03