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Literature summary for 2.7.7.13 extracted from
- The cofactor Mg2+ - a key switch for effective continuous enzymic production of GDP-mannose using recombinant GDP-mannose pyrophosphorylase (1998), Carbohydr. Res., 305, 475-481.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain BL21(DE3)pLysS | Salmonella enterica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ammonium sulfate | - |
Salmonella enterica |  |
| diphosphate | strong product inhibition | Salmonella enterica | |
| GDP-mannose | strong product inhibition | Salmonella enterica | |
| Mg2+ | - |
Salmonella enterica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
Mg2+ | pH 8.0, 25°C | Salmonella enterica | |
| 0.015 | - |
alpha-D-mannose 1-phosphate | pH 8.0, 25°C | Salmonella enterica | |
| 0.04 | - |
GTP | pH 8.0, 25°C | Salmonella enterica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activity depends on the ratio of Mg2+ and GTP, maximum activity at a ratio of about 1 | Salmonella enterica | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + alpha-D-mannose 1-phosphate | Salmonella enterica | - |
GDPmannose + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme, ultrafiltration, Q-Sepharose | Salmonella enterica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, ammonium sulfate suspension, several months, no loss of activity | Salmonella enterica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + alpha-D-mannose 1-phosphate | - |
Salmonella enterica | GDPmannose + diphosphate | - |
? | |
| GTP + alpha-D-mannose 1-phosphate | - |
Salmonella enterica | diphosphate + GDP-mannose | - |
? | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
Mg2+ | pH 8.0, 25°C | Salmonella enterica | |
| 0.009 | - |
GDP-mannose | pH 8.0, 25°C | Salmonella enterica | |
| 0.016 | - |
diphosphate | pH 8.0, 25°C | Salmonella enterica | |
| 0.85 | - |
ammonium sulfate | - |
Salmonella enterica | |
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