KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
mannose 1-phosphate | pH 7.8, 37°C | Sus scrofa | |
0.001 | - |
GDP-alpha-D-mannose | pH 7.8, 37°C | Sus scrofa | |
0.0035 | - |
GTP | pH 7.8, 37°C | Sus scrofa | |
1 | - |
GTP | pH 7.8, 37°C, cosubstrate diphosphate | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
412000 | - |
sucrose velocity sedimentation | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + alpha-D-mannose 1-phosphate | Sus scrofa | - |
GDPmannose + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
blue-Sepharose, DEAE-Sepharose, phenyl-Sepharose, agarose-GTP affinity chromatography | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
thyroid gland | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24.1 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
4°C, stable for a few days, purified enzyme | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dGTP + alpha-D-mannose 1-phosphate | 34% of the activity with GTP | Sus scrofa | dGDPmannose + diphosphate | - |
? | |
GTP + alpha-D-mannose 1-phosphate | - |
Sus scrofa | GDPmannose + diphosphate | - |
r | |
GTP + alpha-D-mannose 1-phosphate | specific for mannose | Sus scrofa | GDPmannose + diphosphate | - |
r | |
GTP + alpha-D-mannose 1-phosphate | no activity with ATP, CTP, TTP, UTP, ADP-alpha-D-glucose, ADP-alpha-D-mannose, CDP-alpha-D-choline, CDP-alpha-D-glucose, CDP-beta-L-fucose, GDP-alpha-D-glucose, dTPP-alpha-D-glucose, IDP-alpha-D-galactose, UDP-alpha-D-glucose, UDP-N-acetyl-alpha-D-glucosamine, UDP-alpha-D-mannose | Sus scrofa | GDPmannose + diphosphate | - |
r | |
ITP + alpha-D-mannose 1-phosphate | 44% of the activity with GTP | Sus scrofa | IDPmannose + diphosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | - |
Sus scrofa |