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Literature summary for 2.7.7.13 extracted from

  • Smoot, J.W.; Serif, G.S.
    Purification of GTP:alpha-D-mannose-1-phosphate guanyltransferase (1985), Eur. J. Biochem., 148, 83-87.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
mannose 1-phosphate pH 7.8, 37°C Sus scrofa
0.001
-
GDP-alpha-D-mannose pH 7.8, 37°C Sus scrofa
0.0035
-
GTP pH 7.8, 37°C Sus scrofa
1
-
GTP pH 7.8, 37°C, cosubstrate diphosphate Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
412000
-
sucrose velocity sedimentation Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + alpha-D-mannose 1-phosphate Sus scrofa
-
GDPmannose + diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
blue-Sepharose, DEAE-Sepharose, phenyl-Sepharose, agarose-GTP affinity chromatography Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
thyroid gland
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24.1
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
4°C, stable for a few days, purified enzyme Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dGTP + alpha-D-mannose 1-phosphate 34% of the activity with GTP Sus scrofa dGDPmannose + diphosphate
-
?
GTP + alpha-D-mannose 1-phosphate
-
Sus scrofa GDPmannose + diphosphate
-
r
GTP + alpha-D-mannose 1-phosphate specific for mannose Sus scrofa GDPmannose + diphosphate
-
r
GTP + alpha-D-mannose 1-phosphate no activity with ATP, CTP, TTP, UTP, ADP-alpha-D-glucose, ADP-alpha-D-mannose, CDP-alpha-D-choline, CDP-alpha-D-glucose, CDP-beta-L-fucose, GDP-alpha-D-glucose, dTPP-alpha-D-glucose, IDP-alpha-D-galactose, UDP-alpha-D-glucose, UDP-N-acetyl-alpha-D-glucosamine, UDP-alpha-D-mannose Sus scrofa GDPmannose + diphosphate
-
r
ITP + alpha-D-mannose 1-phosphate 44% of the activity with GTP Sus scrofa IDPmannose + diphosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7.5
-
Sus scrofa