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Literature summary for 2.7.7.12 extracted from

  • Gross, W.; Schnarrenberger, C.
    Purification and characterization of a galactose-1-phosphate: UDP-glucose uridyltransferase from the red alga Galdieria sulphuraria (1995), Eur. J. Biochem., 234, 258-263.
    View publication on PubMed

General Stability

General Stability Organism
stable to freezing and thawing, purified and crude Galdieria sulphuraria

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by NADH, NAD+, sodium diphosphate Galdieria sulphuraria

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
UDP-glucose pH 8.5, 22°C Galdieria sulphuraria
0.03
-
UDP-galactose pH 7.5, 22°C Galdieria sulphuraria
0.105
-
alpha-D-glucose 1-phosphate pH 7.5, 22°C Galdieria sulphuraria
0.4
-
alpha-D-galactose 1-phosphate pH 8.5, 22°C Galdieria sulphuraria

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
2 * 42000, SDS-PAGE Galdieria sulphuraria
60000
-
gel filtration Galdieria sulphuraria
82000
-
sedimentation equilibrium centrifugation Galdieria sulphuraria

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + alpha-D-galactose 1-phosphate Galdieria sulphuraria involved in conversion of galactose into alpha-D-glucose 1-phosphate, Leloir pathway alpha-D-glucose 1-phosphate + UDP-galactose
-
r

Organism

Organism UniProt Comment Textmining
Galdieria sulphuraria
-
-
-

Purification (Commentary)

Purification (Comment) Organism
about 1800fold to homogeneity Galdieria sulphuraria

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
137
-
purified enzyme Galdieria sulphuraria

Storage Stability

Storage Stability Organism
-20°C, stable Galdieria sulphuraria

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with UDP-xylose, ADP-glucose, and galactose 6-phosphate Galdieria sulphuraria ?
-
?
UDP-glucose + alpha-D-galactose 1-phosphate highly specific for the substrates Galdieria sulphuraria alpha-D-glucose 1-phosphate + UDP-galactose
-
r
UDP-glucose + alpha-D-galactose 1-phosphate involved in conversion of galactose into alpha-D-glucose 1-phosphate, Leloir pathway Galdieria sulphuraria alpha-D-glucose 1-phosphate + UDP-galactose
-
r

Subunits

Subunits Comment Organism
dimer 2 * 42000, SDS-PAGE Galdieria sulphuraria

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at Galdieria sulphuraria

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
activation energy, temperature profile Galdieria sulphuraria

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
half-life: 60 min Galdieria sulphuraria

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9 broad Galdieria sulphuraria