Literature summary for 2.7.7.12 extracted from

Wedekind, J.E.; Frey, P.A.; Rayment, I.
Crystallization and preliminary crystallographic analysis of galactose-1-phosphate uridylyltransferase from Escherichia coli (1994), Acta Crystallogr. Sect. D, 50, 329-331.

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Cloned(Commentary)

Cloned (Comment)	Organism
3 to 4fold overexpression in strain BL21(DE3)	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
17.5-23 mg/ml recombinant protein, hanging drop vapour diffusion method in the presence of 4 mM substrate analog phenyl-UDP, 277 K, 0.1 M sodium succinate, pH 5.9, 0.25 M NaCl, 0.4 M Li2SO4, over 14.5% w/w polyethylene glycol 10000, 1 mM NaN3, 5-6 days, X-ray diffraction structure determination and analysis	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant from overexpressing strain BL21(DE3)	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-glucose + alpha-D-galactose 1-phosphate	-	Escherichia coli	alpha-D-glucose 1-phosphate + UDP-galactose	-	r

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Release 2023.1 (January 2023)