Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.12 extracted from

  • Arabshahi, A.; Brody, R.S.; Smallwood, A.; Tsai, T.C.; Frey, P.A.
    Galactose-1-phosphate uridylyltransferase. Purification of the enzyme and stereochemical course of each step of the double-displacement mechanism (1986), Biochemistry, 25, 5583-5589.
    View publication on PubMed

General Stability

General Stability Organism
PMSF, EDTA and 2-mercaptoethanol stabilize during purification Escherichia coli
purified enzyme has tendency to undergo transition to a lower specific activity form Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + alpha-D-galactose 1-phosphate Escherichia coli
-
alpha-D-glucose 1-phosphate + UDP-galactose
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose double-displacement mechanism Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
238
-
purified enzyme Escherichia coli

Storage Stability

Storage Stability Organism
metal ions or/and substrates do not protect from oxidation or proteolysis during prolonged storage Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alphaS-glucose + alpha-D-galactose 1-phosphate stereochemistry: enzyme accepts (Rp)-UDP-alphaS-glucose converting it to (Rp)-UDP-alphaS-galactose, the overall retention of configuration arises from inversion in each of the two steps Escherichia coli alpha-D-glucose 1-phosphate + UDP-alphaS-galactose (Rp1)-UDP-alphaS-galactose r
UDP-alphaS-glucose + alpha-D-galactose 1-phosphate stereochemistry: enzyme accepts (Rp)-UDP-alphaS-glucose converting it to (Rp)-UDP-alphaS-galactose, the overall retention of configuration arises from inversion in each of the two steps Escherichia coli alpha-D-glucose 1-phosphate + UDP-alphaS-galactose i.e. uridine 5'-(1-thiodiphosphate) galactose r
UDP-alphaS-glucose + alpha-D-galactose 1-phosphate i.e. uridine 5'-(1-thiodiphosphate) glucose Escherichia coli alpha-D-glucose 1-phosphate + UDP-alphaS-galactose (Rp1)-UDP-alphaS-galactose r
UDP-alphaS-glucose + alpha-D-galactose 1-phosphate i.e. uridine 5'-(1-thiodiphosphate) glucose Escherichia coli alpha-D-glucose 1-phosphate + UDP-alphaS-galactose i.e. uridine 5'-(1-thiodiphosphate) galactose r
UDP-alphaS-glucose + alpha-D-galactose 1-phosphate (Rp1)-UDP-alphaS-glucose Escherichia coli alpha-D-glucose 1-phosphate + UDP-alphaS-galactose (Rp1)-UDP-alphaS-galactose r
UDP-alphaS-glucose + alpha-D-galactose 1-phosphate (Rp1)-UDP-alphaS-glucose Escherichia coli alpha-D-glucose 1-phosphate + UDP-alphaS-galactose i.e. uridine 5'-(1-thiodiphosphate) galactose r
UDP-glucose + alpha-D-galactose 1-phosphate
-
Escherichia coli alpha-D-glucose 1-phosphate + UDP-galactose
-
r
UDP-glucose + alpha-D-galactose 1-phosphate stereochemistry: enzyme accepts (Rp)-UDP-alphaS-glucose converting it to (Rp)-UDP-alphaS-galactose, the overall retention of configuration arises from inversion in each of the two steps Escherichia coli alpha-D-glucose 1-phosphate + UDP-galactose
-
r
UDP-glucose + alpha-D-galactose 1-phosphate formation of a covalent uridylyl-enzyme intermediate, i.e. UMP-enzyme Escherichia coli alpha-D-glucose 1-phosphate + UDP-galactose
-
r