General Stability | Organism |
---|---|
PMSF, EDTA and 2-mercaptoethanol stabilize during purification | Escherichia coli |
purified enzyme has tendency to undergo transition to a lower specific activity form | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + alpha-D-galactose 1-phosphate | Escherichia coli | - |
alpha-D-glucose 1-phosphate + UDP-galactose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose | double-displacement mechanism | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
238 | - |
purified enzyme | Escherichia coli |
Storage Stability | Organism |
---|---|
metal ions or/and substrates do not protect from oxidation or proteolysis during prolonged storage | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alphaS-glucose + alpha-D-galactose 1-phosphate | stereochemistry: enzyme accepts (Rp)-UDP-alphaS-glucose converting it to (Rp)-UDP-alphaS-galactose, the overall retention of configuration arises from inversion in each of the two steps | Escherichia coli | alpha-D-glucose 1-phosphate + UDP-alphaS-galactose | (Rp1)-UDP-alphaS-galactose | r | |
UDP-alphaS-glucose + alpha-D-galactose 1-phosphate | stereochemistry: enzyme accepts (Rp)-UDP-alphaS-glucose converting it to (Rp)-UDP-alphaS-galactose, the overall retention of configuration arises from inversion in each of the two steps | Escherichia coli | alpha-D-glucose 1-phosphate + UDP-alphaS-galactose | i.e. uridine 5'-(1-thiodiphosphate) galactose | r | |
UDP-alphaS-glucose + alpha-D-galactose 1-phosphate | i.e. uridine 5'-(1-thiodiphosphate) glucose | Escherichia coli | alpha-D-glucose 1-phosphate + UDP-alphaS-galactose | (Rp1)-UDP-alphaS-galactose | r | |
UDP-alphaS-glucose + alpha-D-galactose 1-phosphate | i.e. uridine 5'-(1-thiodiphosphate) glucose | Escherichia coli | alpha-D-glucose 1-phosphate + UDP-alphaS-galactose | i.e. uridine 5'-(1-thiodiphosphate) galactose | r | |
UDP-alphaS-glucose + alpha-D-galactose 1-phosphate | (Rp1)-UDP-alphaS-glucose | Escherichia coli | alpha-D-glucose 1-phosphate + UDP-alphaS-galactose | (Rp1)-UDP-alphaS-galactose | r | |
UDP-alphaS-glucose + alpha-D-galactose 1-phosphate | (Rp1)-UDP-alphaS-glucose | Escherichia coli | alpha-D-glucose 1-phosphate + UDP-alphaS-galactose | i.e. uridine 5'-(1-thiodiphosphate) galactose | r | |
UDP-glucose + alpha-D-galactose 1-phosphate | - |
Escherichia coli | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
UDP-glucose + alpha-D-galactose 1-phosphate | stereochemistry: enzyme accepts (Rp)-UDP-alphaS-glucose converting it to (Rp)-UDP-alphaS-galactose, the overall retention of configuration arises from inversion in each of the two steps | Escherichia coli | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
UDP-glucose + alpha-D-galactose 1-phosphate | formation of a covalent uridylyl-enzyme intermediate, i.e. UMP-enzyme | Escherichia coli | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r |