Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | requirement | Escherichia coli |
General Stability | Organism |
---|---|
freeze-thawing inactivates purified enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Escherichia coli | |
0.31 | - |
UDP-galactose | pH 8.8, 25°C | Escherichia coli | |
0.41 | - |
UDP-glucose | pH 8.8, 25°C | Escherichia coli | |
0.5 | - |
alpha-D-glucose 1-phosphate | pH 8.8, 25°C | Escherichia coli | |
1.54 | - |
alpha-D-galactose 1-phosphate | pH 8.8, 25°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
2 * 41000, urea/mercaptoethanol treated and S-carboxymethylated enzyme, sedimentation equilibrium centrifugation in 5 M guanidine hydrochloride | Escherichia coli |
80000 | - |
low speed sedimentation equilibrium | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | - |
operator-constitutive mutant strain C81-2 | - |
Purification (Comment) | Organism |
---|---|
to homogeneity | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
209 | - |
purified enzyme | Escherichia coli |
Storage Stability | Organism |
---|---|
0°C, 10% loss of activity per week | Escherichia coli |
0°C, 5 mM potassium phosphate buffer, pH 7, 20 mg/ml bovine serum albumin, 5 h stable | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with fructose 1-phosphate and galactose 6-phosphate | Escherichia coli | ? | - |
? | |
additional information | no activity with ADP-glucose or GDP-glucose | Escherichia coli | ? | - |
? | |
additional information | no activity with ribose 1-phosphate and mannose 6-phosphate | Escherichia coli | ? | - |
? | |
TDP-glucose + alpha-D-galactose 1-phosphate | no activity | Escherichia coli | alpha-D-glucose 1-phosphate + TDP-galactose | - |
? | |
UDP-glucose + alpha-D-galactose 1-phosphate | highly specific for the substrates | Escherichia coli | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41000, urea/mercaptoethanol treated and S-carboxymethylated enzyme, sedimentation equilibrium centrifugation in 5 M guanidine hydrochloride | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
activation energy, temperature profile | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
278 | - |
alpha-D-galactose 1-phosphate | pH 8.8, 25°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 9.8 | about half-maximal activity at pH 7.2 and about 70% of maximal activity at pH 9.8 | Escherichia coli |