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Literature summary for 2.7.7.12 extracted from

  • Saito, S.; Ozutsumi, M.; Kurahashi, K.
    Galactose 1-phosphate uridylyltransferase of Escherichia coli. II. Further purification and characterization (1967), J. Biol. Chem., 242, 2362-2368.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cysteine requirement Escherichia coli

General Stability

General Stability Organism
freeze-thawing inactivates purified enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Escherichia coli
0.31
-
UDP-galactose pH 8.8, 25°C Escherichia coli
0.41
-
UDP-glucose pH 8.8, 25°C Escherichia coli
0.5
-
alpha-D-glucose 1-phosphate pH 8.8, 25°C Escherichia coli
1.54
-
alpha-D-galactose 1-phosphate pH 8.8, 25°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
2 * 41000, urea/mercaptoethanol treated and S-carboxymethylated enzyme, sedimentation equilibrium centrifugation in 5 M guanidine hydrochloride Escherichia coli
80000
-
low speed sedimentation equilibrium Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli
-
operator-constitutive mutant strain C81-2
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
209
-
purified enzyme Escherichia coli

Storage Stability

Storage Stability Organism
0°C, 10% loss of activity per week Escherichia coli
0°C, 5 mM potassium phosphate buffer, pH 7, 20 mg/ml bovine serum albumin, 5 h stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with fructose 1-phosphate and galactose 6-phosphate Escherichia coli ?
-
?
additional information no activity with ADP-glucose or GDP-glucose Escherichia coli ?
-
?
additional information no activity with ribose 1-phosphate and mannose 6-phosphate Escherichia coli ?
-
?
TDP-glucose + alpha-D-galactose 1-phosphate no activity Escherichia coli alpha-D-glucose 1-phosphate + TDP-galactose
-
?
UDP-glucose + alpha-D-galactose 1-phosphate highly specific for the substrates Escherichia coli alpha-D-glucose 1-phosphate + UDP-galactose
-
r

Subunits

Subunits Comment Organism
dimer 2 * 41000, urea/mercaptoethanol treated and S-carboxymethylated enzyme, sedimentation equilibrium centrifugation in 5 M guanidine hydrochloride Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
activation energy, temperature profile Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
278
-
alpha-D-galactose 1-phosphate pH 8.8, 25°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7.2 9.8 about half-maximal activity at pH 7.2 and about 70% of maximal activity at pH 9.8 Escherichia coli