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Literature summary for 2.7.7.12 extracted from

  • Tamada, Y.; Swanson, B.A.; Arabshahi, A.; Frey, P.A.
    Preparation and characterization of a bifunctional fusion enzyme composed of UDP-galactose 4-epimerase and galactose-1-P uridylyltransferase (1994), Bioconjug. Chem., 5, 660-665.
    View publication on PubMed
Search for more literature for 2.7.7.12

Cloned(Commentary)

Cloned (Comment) Organism
construction and overexpression of bifunctional fusion protein composed of galactose-2-phosphate uridylyltransferase and UDP-galactose 4-epimerase with an intervening linker of 3 Ala residues Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information construction and expression of bifunctional fusion protein composed of galactose-2-phosphate uridylyltransferase and UDP-galactose 4-epimerase with an intervening linker of 3 Ala residues, 20% increased Vmax compared to a mixture of the single enzymes Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 UDP-glucose purified recombinant bifunctional chimeric fusion protein, pH 8.5, 27°C Escherichia coli
0.29
-
 alpha-D-galactose 1-phosphate purified recombinant bifunctional chimeric fusion protein, pH 8.5, 27°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 the recombinant chimeric fusion protein exists as monomer, dimer and tetramer, all are active Escherichia coli
80000
-
 recombinant bifunctional chimeric fusion protein, monomer, gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 galT gene
-

Purification (Commentary)

Purification (Comment) Organism
recombinant bifunctional fusion protein from strain BL21(DE3), pLysS, pT7ET to near homogeneity, 9fold Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1080
-
 purified recombinant bifunctional chimeric fusion protein Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + alpha-D-galactose 1-phosphate recombinant bifunctional chimeric fusion protein Escherichia coli alpha-D-glucose 1-phosphate + UDP-galactose
-
 r

Subunits

Subunits Comment Organism
More the recombinant chimeric fusion protein exists as monomer, dimer and tetramer, all are active Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
24
-
 alpha-D-galactose 1-phosphate purified recombinant bifunctional chimeric fusion protein, pH 8.5, 27°C Escherichia coli