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Literature summary for 2.7.7.101 extracted from
- Primase, the dnaG protein of Escherichia coli. An enzyme which starts DNA chains (1978), J. Biol. Chem., 253, 758-764 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | - |
Escherichia coli |  |
| phosphate | 0.04 mM, 40% inhibition | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
rNTP | pH 7.5, 30°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | presence of Mg2+ required, optimum concentration 2-8 mM | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0ABS5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.09 | - |
pH 7.5, 30°C | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -180°C, stable for at least 1 year | Escherichia coli |
| 0°C, stable for months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bacteriophage G4 ssDNA + n rNTP | - |
Escherichia coli | bacteriophage G4 ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate | - |
? | |
| additional information | other single-stranded and duplex DNA templates tested are inactive | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 37 | - |
Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
about 50% of maximum activity | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | - |
Escherichia coli |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 50 | - |
pH 7.5, 30°C | Escherichia coli | NaCl | |
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Release 2023.1 (January 2023)
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