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Literature summary for 2.7.7.1 extracted from

  • Kato, M.; Lin, S.J.
    YCL047C/POF1 is a novel nicotinamide mononucleotide adenylyltransferase (NMNAT) in Saccharomyces cerevisiae (2014), J. Biol. Chem., 289, 15577-15587.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.1

Cloned(Commentary)

Cloned (Comment) Organism
gene Pof1 or YCL047C, DNA and amino acid sequence determination and analysis, sequence comparisons, construction of the POF1-URA3 episomal plasmid (pRS316-POF1) derived from pADH1-POF1, which is constructed in the integrative pPP81 (LEU2) vector, recombinant expression of His-tagged enzyme from plasmid pET28-POF1 in Escherichia coli strain Rosetta (DE3), quantitative PCR expression analysis Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information construction of a haploid single gene deletion mutant, cells lacking the ORF YCL047C/POF1 display strong cross-feeding activity Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.26
-
 nicotinamide ribonucleotide pH 10.0, 30°C, recombinant enzyme Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + nicotinamide ribonucleotide Saccharomyces cerevisiae
-
 diphosphate + NAD+
-
 ?
ATP + nicotinamide ribonucleotide Saccharomyces cerevisiae BY4742
-
 diphosphate + NAD+
-
 ?
additional information Saccharomyces cerevisiae the amino acid sequence of Pof1 indicates that it is a putative nicotinamide mononucleotide adenylyltransferase (NMNAT). Unlike other yeast NMNATs, Pof1 exhibits NMN-specific adenylyltransferase activity ?
-
 ?
additional information Saccharomyces cerevisiae BY4742 the amino acid sequence of Pof1 indicates that it is a putative nicotinamide mononucleotide adenylyltransferase (NMNAT). Unlike other yeast NMNATs, Pof1 exhibits NMN-specific adenylyltransferase activity ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P25576 gene Pof1 or YCL047C
-
Saccharomyces cerevisiae BY4742 P25576 gene Pof1 or YCL047C
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + nicotinamide ribonucleotide
-
 Saccharomyces cerevisiae diphosphate + NAD+
-
 ?
ATP + nicotinamide ribonucleotide
-
 Saccharomyces cerevisiae BY4742 diphosphate + NAD+
-
 ?
additional information the amino acid sequence of Pof1 indicates that it is a putative nicotinamide mononucleotide adenylyltransferase (NMNAT). Unlike other yeast NMNATs, Pof1 exhibits NMN-specific adenylyltransferase activity Saccharomyces cerevisiae ?
-
 ?
additional information the amino acid sequence of Pof1 indicates that it is a putative nicotinamide mononucleotide adenylyltransferase (NMNAT). Unlike other yeast NMNATs, Pof1 exhibits NMN-specific adenylyltransferase activity Saccharomyces cerevisiae BY4742 ?
-
 ?

Synonyms

Synonyms Comment Organism
mononucleotide adenylyltransferase
-
 Saccharomyces cerevisiae
NMNAT
-
 Saccharomyces cerevisiae
Pof1
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
35.33
-
 nicotinamide ribonucleotide pH 10.0, 30°C, recombinant enzyme Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
 assay at Saccharomyces cerevisiae

Expression

Organism Comment Expression
Saccharomyces cerevisiae expression of POF1 is slightly induced in the late log phase up

General Information

General Information Comment Organism
malfunction deletion of POF1 significantly lowers NAD+ levels and decreases the efficiency of nicotinamide riboside utilization, resistance to oxidative stress, and nicotinamide riboside-induced life span extension Saccharomyces cerevisiae