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Literature summary for 2.7.7.1 extracted from

  • Kusumanchi, P.; Zhang, Y.; Jani, M.B.; Jayaram, N.H.; Khan, R.A.; Tang, Y.; Antony, A.C.; Jayaram, H.N.
    Nicotinamide mononucleotide adenylyltransferase2 overexpression enhances colorectal cancer cell-kill by Tiazofurin (2013), Cancer Gene Ther., 20, 403-412.
    View publication on PubMed
Search for more literature for 2.7.7.1

Cloned(Commentary)

Cloned (Comment) Organism
gene NMNAT2, transfection of hNMNAT2 results in a six- and threefold cytoplasmic overexpression in Caco-2 and HT-29 cell lines, respectively, correlating with Tiazofurin-induced enhanced cell killing Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + nicotinamide ribonucleotide Homo sapiens
-
 diphosphate + NAD+
-
 r
ATP + tiazofurin Homo sapiens tiazofurin is a pro-drug that is metabolized by cytosolic nicotinamide mononucleotide adenylyltransferase2 diphosphate + thiazole-4-carboxamide adenine dinucleotide
-
 ?
additional information Homo sapiens bifunctional enzyme, that also shows nicotinate-nucleotide adenylyltransferase activity, EC 2.7.7.18 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9BZQ4 gene NMNAT2
-

Source Tissue

Source Tissue Comment Organism Textmining
Caco-2 cell
-
 Homo sapiens
-
colorectal cancer cell exhibits low levels of NMNAT2 Homo sapiens
-
HT-29 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + nicotinamide ribonucleotide
-
 Homo sapiens diphosphate + NAD+
-
 r
ATP + tiazofurin tiazofurin is a pro-drug that is metabolized by cytosolic nicotinamide mononucleotide adenylyltransferase2 Homo sapiens diphosphate + thiazole-4-carboxamide adenine dinucleotide
-
 ?
additional information bifunctional enzyme, that also shows nicotinate-nucleotide adenylyltransferase activity, EC 2.7.7.18 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
nicotinamide mononucleotide adenylyltransferase2
-
 Homo sapiens
NMNAT2
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction colorectal cancer cells, that exhibit low levels of NMNAT2 and are refractory to tiazofurin, can be rendered sensitive to Tiazofurin by overexpressing NMNAT2 Homo sapiens
physiological function tiazofurin is a pro-drug that is metabolized by cytosolic nicotinamide mononucleotide adenylyltransferase2 (NMNAT2) to thiazole-4-carboxamide adenine dinucleotide, a potent inhibitor of inosine 5'-monophosphate dehydrogenase required for cellular guanylate synthesis. Resistance of colorectal cancer cell-kill to Tiazofurin can be overcome by sequentially overexpressing hNMNAT2 and then facilitating the uptake of Tiazofurin by folate-tethered nanoparticles, which enter cells via folate receptors Homo sapiens