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Literature summary for 2.7.7.1 extracted from

  • Orsomando, G.; Cialabrini, L.; Amici, A.; Mazzola, F.; Ruggieri, S.; Conforti, L.; Janeckova, L.; Coleman, M.P.; Magni, G.
    Simultaneous single-sample determination of NMNAT isozyme activities in mouse tissues (2012), PLoS ONE, 7, e53271.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0252
-
 nicotinamide ribonucleotide isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
0.0335
-
 ATP isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
0.0385
-
 nicotinamide ribonucleotide isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
0.039
-
 ATP isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
0.082
-
 ATP isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
0.1176
-
 nicotinamide ribonucleotide isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ isoform NMNAT3 is predominantly activated by Co2+ ions (5 mM) Mus musculus
Mg2+ isoform NMNAT2 is predominantly activated by Mg2+ ions (0.5 mM) Mus musculus
Zn2+ isoform NMNAT1 is predominantly activated by Zn2+ ions (0.05 mM) Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q5HZI3 isoform NMNAT2
-
Mus musculus Q99JR6 isoform NMNAT3
-
Mus musculus Q9EPA7 isoform NMNAT1
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity chromatography Mus musculus
Talon affinity column chromatography Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Mus musculus
-
liver
-
 Mus musculus
-

Storage Stability

Storage Stability Organism
-20°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 20% loss of activity Mus musculus
-20°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 55% loss of activity Mus musculus
-20°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 60% loss of activity Mus musculus
4°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 55% loss of activity Mus musculus
4°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 60% loss of activity Mus musculus
4°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 90% loss of activity Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + nicotinamide ribonucleotide
-
 Mus musculus diphosphate + NAD+
-
 r
ATP + nicotinic acid mononucleotide
-
 Mus musculus diphosphate + deamido-NAD+
-
 r
GTP + nicotinamide ribonucleotide
-
 Mus musculus ?
-
 ?
ITP + nicotinamide ribonucleotide
-
 Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
nicotinamide mononucleotide adenylyltransferase
-
 Mus musculus
NMNAT1 isoform Mus musculus
NMNAT2 isoform Mus musculus
NMNAT3 isoform Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.74
-
 ATP isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
0.75
-
 nicotinamide ribonucleotide isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
2.33
-
 ATP isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
2.87
-
 nicotinamide ribonucleotide isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
15
-
 ATP isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
15.5
-
 nicotinamide ribonucleotide isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10
-
 nicotinamide ribonucleotide isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
20
-
 ATP isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
30
-
 ATP isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
70
-
 nicotinamide ribonucleotide isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
450
-
 ATP isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
610
-
 nicotinamide ribonucleotide isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus