Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | SO42- has no effect | Methanocaldococcus jannaschii | |
SO42- | 0.1 M, 20fold activation | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP-ribose | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
NAD+ | isoform yNMNAT-2 | Saccharomyces cerevisiae | |
0.073 | - |
NAD+ | isoform yNMNAT-1 | Saccharomyces cerevisiae | |
0.083 | - |
diphosphate | isoform yNMNAT-1 | Saccharomyces cerevisiae | |
0.11 | - |
nicotinamide ribonucleotide | isoform yNMNAT-1 | Saccharomyces cerevisiae | |
0.13 | - |
nicotinamide ribonucleotide | isoform yNMNAT-2 | Saccharomyces cerevisiae | |
0.19 | - |
ATP | isoform yNMNAT-1 | Saccharomyces cerevisiae | |
1.4 | - |
ATP | isoform yNMNAT-2 | Saccharomyces cerevisiae | |
5 | - |
diphosphate | isoform yNMNAT-2 | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | metal ion required. Order of decreasing effectiveness: Co2+, Ni2+, Mg2+, Zn2+, Mn2+, isoform yNMNAT-2 | Saccharomyces cerevisiae | |
Co2+ | metal ion required. Order of decreasing effectiveness: Ni2+, Zn2+, Co2+, Mg2+, Mn2+, isoform yNMNAT-1 | Saccharomyces cerevisiae | |
Mg2+ | metal ion required. Order of decreasing effectiveness: Co2+, Ni2+, Mg2+, Zn2+, Mn2+, isoform yNMNAT-2 | Saccharomyces cerevisiae | |
Mg2+ | metal ion required. Order of decreasing effectiveness: Ni2+, Zn2+, Co2+, Mg2+, Mn2+, isoform yNMNAT-1 | Saccharomyces cerevisiae | |
Mn2+ | metal ion required. Order of decreasing effectiveness: Co2+, Ni2+, Mg2+, Zn2+, Mn2+, isoform yNMNAT-2 | Saccharomyces cerevisiae | |
Mn2+ | metal ion required. Order of decreasing effectiveness: Ni2+, Zn2+, Co2+, Mg2+, Mn2+, isoform yNMNAT-1 | Saccharomyces cerevisiae | |
Ni2+ | metal ion required. Order of decreasing effectiveness: Co2+, Ni2+, Mg2+, Zn2+, Mn2+, isoform yNMNAT-2 | Saccharomyces cerevisiae | |
Ni2+ | metal ion required. Order of decreasing effectiveness: Ni2+, Zn2+, Co2+, Mg2+, Mn2+, isoform yNMNAT-1 | Saccharomyces cerevisiae | |
Zn2+ | metal ion required. Order of decreasing effectiveness: Co2+, Ni2+, Mg2+, Zn2+, Mn2+, isoform yNMNAT-2 | Saccharomyces cerevisiae | |
Zn2+ | metal ion required. Order of decreasing effectiveness: Ni2+, Zn2+, Co2+, Mg2+, Mn2+, isoform yNMNAT-1 | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18600 | - |
x * 18600, SDS-PAGE | Saccharolobus solfataricus |
19600 | - |
x * 19600, SDS-PAGE | Methanocaldococcus jannaschii |
46000 | - |
4 * 46000, isoform yNMNAT-2, SDS-PAGE | Saccharomyces cerevisiae |
48000 | - |
4 * 48000, isoform yNMNAT-1, SDS-PAGE | Saccharomyces cerevisiae |
66000 | - |
- |
Saccharolobus solfataricus |
72000 | - |
- |
Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nicotinamide ribonucleotide | Haemophilus influenzae | final step in biosynthesis of NAD+ | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | Bacillus subtilis | final step in biosynthesis of NAD+ | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | Homo sapiens | final step in biosynthesis of NAD+ | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | Saccharomyces cerevisiae | final step in biosynthesis of NAD+ | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | Bos taurus | final step in biosynthesis of NAD+ | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | Methanothermobacter thermautotrophicus | final step in biosynthesis of NAD+ | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | Saccharolobus solfataricus | final step in biosynthesis of NAD+ | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | Methanocaldococcus jannaschii | final step in biosynthesis of NAD+ | diphosphate + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bos taurus | - |
- |
- |
Haemophilus influenzae | - |
- |
- |
Homo sapiens | - |
- |
- |
Methanocaldococcus jannaschii | - |
- |
- |
Methanothermobacter thermautotrophicus | - |
- |
- |
Saccharolobus solfataricus | - |
- |
- |
Saccharomyces cerevisiae | - |
isoform yNMNAT-1 | - |
Saccharomyces cerevisiae | - |
isoform yNMNAT-2 | - |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
corpus luteum | activity is high during mid luteal phase, when corpora lutea are mature with respect to structure and function, while it dramatically decreases following PGF2alpha analog injection | Bos taurus | - |
placenta | - |
Homo sapiens | - |
testis | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nicotinamide ribonucleotide | - |
Haemophilus influenzae | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | - |
Bacillus subtilis | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | - |
Homo sapiens | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | - |
Bos taurus | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | - |
Methanothermobacter thermautotrophicus | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | - |
Saccharolobus solfataricus | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | - |
Methanocaldococcus jannaschii | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | final step in biosynthesis of NAD+ | Haemophilus influenzae | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | final step in biosynthesis of NAD+ | Bacillus subtilis | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | final step in biosynthesis of NAD+ | Homo sapiens | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | final step in biosynthesis of NAD+ | Saccharomyces cerevisiae | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | final step in biosynthesis of NAD+ | Bos taurus | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | final step in biosynthesis of NAD+ | Methanothermobacter thermautotrophicus | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | final step in biosynthesis of NAD+ | Saccharolobus solfataricus | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | final step in biosynthesis of NAD+ | Methanocaldococcus jannaschii | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | isoform yNMNAT-1 | Saccharomyces cerevisiae | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | isoform yNMNAT-2 | Saccharomyces cerevisiae | diphosphate + NAD+ | - |
? | |
CTP + nicotinamide ribonucleotide | isoform yNMNAT-2 | Saccharomyces cerevisiae | diphosphate + nicotinamide cytosine dinucleotide | - |
? | |
dATP + nicotinamide ribonucleotide | isoform yNMNAT-1 | Saccharomyces cerevisiae | diphosphate + ? | - |
? | |
dATP + nicotinamide ribonucleotide | isoform yNMNAT-2 | Saccharomyces cerevisiae | diphosphate + nicotinamide deoxyadenosine dinucleotide | - |
? | |
GTP + nicotinamide ribonucleotide | isoform yNMNAT-2 | Saccharomyces cerevisiae | diphosphate + nicotinamide guanine dinucleotide | - |
? | |
ITP + nicotinamide ribonucleotide | isoform yNMNAT-2 | Saccharomyces cerevisiae | diphosphate + nicotinamide hypoxanthine dinucleotide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 18600, SDS-PAGE | Saccharolobus solfataricus |
? | x * 19600, SDS-PAGE | Methanocaldococcus jannaschii |
hexamer | - |
Methanothermobacter thermautotrophicus |
hexamer | - |
Methanocaldococcus jannaschii |
tetramer | - |
Haemophilus influenzae |
tetramer | 4 * 46000, isoform yNMNAT-2, SDS-PAGE | Saccharomyces cerevisiae |
tetramer | 4 * 48000, isoform yNMNAT-1, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
hNMNAT-1 | - |
Homo sapiens |
nicotinamide mononucleotide adenylyltransferase | - |
Haemophilus influenzae |
nicotinamide mononucleotide adenylyltransferase | - |
Bacillus subtilis |
nicotinamide mononucleotide adenylyltransferase | - |
Homo sapiens |
nicotinamide mononucleotide adenylyltransferase | - |
Saccharomyces cerevisiae |
nicotinamide mononucleotide adenylyltransferase | - |
Bos taurus |
nicotinamide mononucleotide adenylyltransferase | - |
Methanothermobacter thermautotrophicus |
nicotinamide mononucleotide adenylyltransferase | - |
Saccharolobus solfataricus |
nicotinamide mononucleotide adenylyltransferase | - |
Methanocaldococcus jannaschii |
NMNAT | - |
Haemophilus influenzae |
NMNAT | - |
Bacillus subtilis |
NMNAT | - |
Saccharomyces cerevisiae |
NMNAT | - |
Bos taurus |
NMNAT | - |
Methanothermobacter thermautotrophicus |
NMNAT | - |
Saccharolobus solfataricus |
NMNAT | - |
Methanocaldococcus jannaschii |
yNMNAT-1 | - |
Saccharomyces cerevisiae |
yNMNAT-2 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
above | Methanocaldococcus jannaschii |
97 | - |
above | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-life is 30 min | Saccharolobus solfataricus |
90 | - |
half-life is 1 h | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | 7.8 | - |
Saccharolobus solfataricus |
6.5 | 8 | isoform yNMNAT-2 | Saccharomyces cerevisiae |
7 | 8.5 | isoform yNMNAT-1 | Saccharomyces cerevisiae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharomyces cerevisiae | 4.2, 4.9 and 6.2, isoform yNMNAT-1 | - |
4.2 |
Saccharomyces cerevisiae | predicted, isoform yNMNAT-2 | - |
5.6 |