Activating Compound | Comment | Organism | Structure |
---|---|---|---|
SO42- | at 0.1 M activation | Saccharolobus solfataricus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | most effective, 0.5 mM giving the same extent of activation like 10 mM Mg2+ | Methanocaldococcus jannaschii | |
Cu2+ | - |
Methanocaldococcus jannaschii | |
Fe3+ | - |
Methanocaldococcus jannaschii | |
Mg2+ | - |
Saccharolobus solfataricus | |
Mg2+ | - |
Methanocaldococcus jannaschii | |
Mn2+ | - |
Methanocaldococcus jannaschii | |
Ni2+ | most effective, 0.5 mM giving the same extent of activation like 10 mM Mg2+ | Methanocaldococcus jannaschii | |
Zn2+ | - |
Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18600 | - |
x * 18600, SDS-PAGE | Saccharolobus solfataricus |
19600 | - |
x * 19600, SDS-PAGE | Methanocaldococcus jannaschii |
66000 | - |
gel filtration | Saccharolobus solfataricus |
72000 | - |
gel filtration | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.8 | - |
- |
Saccharolobus solfataricus |
187 | - |
- |
Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nicotinamide ribonucleotide | i.e. NMN or nicotinamide mononucleotide | Saccharolobus solfataricus | diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | i.e. NMN or nicotinamide mononucleotide | Methanocaldococcus jannaschii | diphosphate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 18600, SDS-PAGE | Saccharolobus solfataricus |
? | x * 19600, SDS-PAGE | Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
stable for 6 h | Methanocaldococcus jannaschii |
80 | - |
half life: 3 h | Methanocaldococcus jannaschii |
90 | - |
half life: 1 h | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | 7.8 | - |
Saccharolobus solfataricus |