Literature summary for 2.7.7.1 extracted from

Magni, G.; Natalini, P.; Santarelli, I.; Vita, A.; Raffaelli, N.; Ruggieri, S.
NAD pyrophosphorylase from yeast chromatin. Purification and properties (1987), Basic Appl. Histochem., 31, 255-271.

Search for more literature for 2.7.7.1

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000019	-	NMN	-	Saccharomyces cerevisiae
0.11	-	ATP	-	Saccharomyces cerevisiae
0.147	0.2	nicotinamide ribonucleotide	-	Saccharomyces cerevisiae
5	-	nicotinate ribonucleotide	-	Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	alpha4, 4 * 50000, SDS-PAGE, with or without 2-mercaptoethanol	Saccharomyces cerevisiae
200000	-	gel filtration	Saccharomyces cerevisiae
200000	-	sucrose density gradient centrifugation	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + nicotinamide ribonucleotide	Saccharomyces cerevisiae	-	diphosphate + NAD+	-	r

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	baker's yeast	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	possibly ADP-ribosylated	Saccharomyces cerevisiae
glycoprotein	2% carbohydrates	Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + nicotinamide ribonucleotide	-	Saccharomyces cerevisiae	diphosphate + NAD+	-	r
ATP + nicotinamide ribonucleotide	i.e. NMN or nicotinamide mononucleotide	Saccharomyces cerevisiae	diphosphate + NAD+	-	r

Subunits

Subunits	Comment	Organism
tetramer	alpha4, 4 * 50000, SDS-PAGE, with or without 2-mercaptoethanol	Saccharomyces cerevisiae

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)