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Literature summary for 2.7.7.1 extracted from

  • Natalini, P.; Ruggieri, S.; Raffaelli, N.; Magni, G.
    Nicotinamide mononucleotide adenylyltransferase. Molecular and enzymatic properties of the homogeneous enzyme from bakers yeast (1986), Biochemistry, 25, 3725-3729.
    View publication on PubMed
Search for more literature for 2.7.7.1

General Stability

General Stability Organism
bovine serum albumin stabilizes Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
diphosphate product inhibition Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000019
-
 NMN 37°C, pH 7.6 Saccharomyces cerevisiae
0.11
-
 ATP 37°C, pH 7.6 Saccharomyces cerevisiae
0.147 0.2 nicotinamide ribonucleotide 37°C, pH 7.6 Saccharomyces cerevisiae
5
-
 nicotinate ribonucleotide 37°C, pH 7.6 Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid composition Saccharomyces cerevisiae
50000
-
 alpha4, 4 * 50000, SDS-PAGE, with or without 2-mercaptoethanol Saccharomyces cerevisiae
200000
-
 gel filtration Saccharomyces cerevisiae
200000
-
 sucrose density gradient centrifugation Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + nicotinamide ribonucleotide Saccharomyces cerevisiae
-
 diphosphate + NAD+
-
 r

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 baker's yeast
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 2% carbohydrates Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8
-
 purification step: Matrix Gel Green A Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-20°C, in 20 mM HEPES buffer, pH 7.5, about 20% loss of activity within 1 month Saccharomyces cerevisiae
4°C, in 20 mM HEPES buffer, pH 7.5, about 30% loss of activity within 2 weeks Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + nicotinamide ribonucleotide
-
 Saccharomyces cerevisiae diphosphate + NAD+
-
 r
ATP + nicotinamide ribonucleotide best substrate Saccharomyces cerevisiae diphosphate + NAD+
-
 r
ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Saccharomyces cerevisiae diphosphate + NAD+
-
 r
ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Saccharomyces cerevisiae diphosphate + nicotinic acid-adenine dinucleotide
-
 r
ATP + nicotinate ribonucleotide less efficient Saccharomyces cerevisiae diphosphate + nicotinic acid-adenine dinucleotide
-
 r

Subunits

Subunits Comment Organism
tetramer alpha4, 4 * 50000, SDS-PAGE, with or without 2-mercaptoethanol Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.4
-
 Saccharomyces cerevisiae

pI Value

Organism Comment pI Value Maximum pI Value
Saccharomyces cerevisiae in the presence of 4 M urea, multiple pIs in its absence at pH 4.2, 4.9 and 6.2
-
 6.2