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Literature summary for 2.7.6.5 extracted from

  • Weiss, L.A.; Stallings, C.L.
    Essential roles for Mycobacterium tuberculosis Rel beyond the production of (p)ppGpp (2013), J. Bacteriol., 195, 5629-5638.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.6.5

Protein Variants

Protein Variants Comment Organism
H344Y site-directed mutagenesis, the mutant enzyme shows no synthase activity Mycobacterium tuberculosis
H80A site-directed mutagenesis, the mutant enzyme shows no hydrolase activity Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + GDP Mycobacterium tuberculosis
-
 AMP + guanosine 3'-diphosphate 5'-diphosphate
-
 ?
ATP + GDP Mycobacterium tuberculosis ATCC 25618
-
 AMP + guanosine 3'-diphosphate 5'-diphosphate
-
 ?
ATP + GTP Mycobacterium tuberculosis
-
 AMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?
ATP + GTP Mycobacterium tuberculosis ATCC 25618
-
 AMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?
guanosine 3'-diphosphate 5'-diphosphate + H2O Mycobacterium tuberculosis
-
 GDP + diphosphate
-
 ?
guanosine 3'-diphosphate 5'-diphosphate + H2O Mycobacterium tuberculosis ATCC 25618
-
 GDP + diphosphate
-
 ?
guanosine 3'-diphosphate 5'-triphosphate + H2O Mycobacterium tuberculosis
-
 GTP + diphosphate
-
 ?
guanosine 3'-diphosphate 5'-triphosphate + H2O Mycobacterium tuberculosis ATCC 25618
-
 GTP + diphosphate
-
 ?
additional information Mycobacterium tuberculosis the enzyme transfers a diphosphate from ATP to GDP or GTP to synthesize guanosine 3'-diphosphate 5'-diphosphate (ppGpp) and guanosine 3'-diphosphate 5'-triphosphate (pppGpp), respectively. Enzyme RelMtb also encodes a second, distinct catalytic domain that hydrolyzes (p)ppGpp into diphosphate and GDP or GTP ?
-
 ?
additional information Mycobacterium tuberculosis ATCC 25618 the enzyme transfers a diphosphate from ATP to GDP or GTP to synthesize guanosine 3'-diphosphate 5'-diphosphate (ppGpp) and guanosine 3'-diphosphate 5'-triphosphate (pppGpp), respectively. Enzyme RelMtb also encodes a second, distinct catalytic domain that hydrolyzes (p)ppGpp into diphosphate and GDP or GTP ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WHG9 gene relA
-
Mycobacterium tuberculosis ATCC 25618 P9WHG9 gene relA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + GDP
-
 Mycobacterium tuberculosis AMP + guanosine 3'-diphosphate 5'-diphosphate
-
 ?
ATP + GDP
-
 Mycobacterium tuberculosis ATCC 25618 AMP + guanosine 3'-diphosphate 5'-diphosphate
-
 ?
ATP + GTP
-
 Mycobacterium tuberculosis AMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?
ATP + GTP
-
 Mycobacterium tuberculosis ATCC 25618 AMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?
guanosine 3'-diphosphate 5'-diphosphate + H2O
-
 Mycobacterium tuberculosis GDP + diphosphate
-
 ?
guanosine 3'-diphosphate 5'-diphosphate + H2O
-
 Mycobacterium tuberculosis ATCC 25618 GDP + diphosphate
-
 ?
guanosine 3'-diphosphate 5'-triphosphate + H2O
-
 Mycobacterium tuberculosis GTP + diphosphate
-
 ?
guanosine 3'-diphosphate 5'-triphosphate + H2O
-
 Mycobacterium tuberculosis ATCC 25618 GTP + diphosphate
-
 ?
additional information the enzyme transfers a diphosphate from ATP to GDP or GTP to synthesize guanosine 3'-diphosphate 5'-diphosphate (ppGpp) and guanosine 3'-diphosphate 5'-triphosphate (pppGpp), respectively. Enzyme RelMtb also encodes a second, distinct catalytic domain that hydrolyzes (p)ppGpp into diphosphate and GDP or GTP Mycobacterium tuberculosis ?
-
 ?
additional information the enzyme transfers a diphosphate from ATP to GDP or GTP to synthesize guanosine 3'-diphosphate 5'-diphosphate (ppGpp) and guanosine 3'-diphosphate 5'-triphosphate (pppGpp), respectively. Enzyme RelMtb also encodes a second, distinct catalytic domain that hydrolyzes (p)ppGpp into diphosphate and GDP or GTP Mycobacterium tuberculosis ATCC 25618 ?
-
 ?

Synonyms

Synonyms Comment Organism
(p)ppGpp synthetase
-
 Mycobacterium tuberculosis
Rel
-
 Mycobacterium tuberculosis
RelA
-
 Mycobacterium tuberculosis
RelMtb
-
 Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mycobacterium tuberculosis

General Information

General Information Comment Organism
malfunction Mycobacterium tuberculosis strains expressing the synthetase-dead RelMtb H344Y mutant do not persist in mice. Deletion of a second predicted (p)ppGpp synthetase has no effect on pathogenesis, demonstrating. Expression of an allele encoding the hydrolase-dead RelMtb mutant, RelMtb H80A, decreases the growth rate of Mycobacterium tuberculosis and changes the colony morphology of the bacteria. RelMtb H80A expression during acute or chronic Mycobacterium tuberculosis infection in mice is lethal to the infecting bacteria. Phenotypes, overview Mycobacterium tuberculosis
physiological function in Mycobacterium tuberculosis, the stringent response to amino acid starvation is mediated by the enzyme Rel (RelMtb), which transfers a diphosphate from ATP to GDP or GTP to synthesize guanosine 3'-diphosphate 5'-diphosphate (ppGpp) and guanosine 3'-diphosphate 5'-triphosphate (pppGpp), respectively. (p)ppGpp then influences numerous metabolic processes. RelMtb also encodes a second, distinct catalytic domain that hydrolyzes (p)ppGpp into diphosphate and GDP or GTP. RelMtb is required for chronic Mycobacterium tuberculosis infection in C57BL/6 mice. The RelMtb (p)ppGpp synthetase activity is required for maintaining bacterial titers during chronic infection, RelMtb is the major contributor to (p)ppGpp production during infection, RelMtb (p)ppGpp hydrolase activity is essential for acute and chronic infection of mice Mycobacterium tuberculosis