Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 5'-(beta,gamma-Imino)triphosphate | - |
Brevibacillus brevis | |
dGDP | - |
Brevibacillus brevis | |
dGTP | - |
Brevibacillus brevis | |
Mg2+ | required as a counter ion for the nucleoside substrate, excess of Mg2+ is inhibitory | Brevibacillus brevis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
GDP | 30°C, pH 8.1 | Brevibacillus brevis | |
0.62 | - |
ATP | cosubstrate GDP, pH 8.1, 30°C | Brevibacillus brevis | |
0.71 | - |
ATP | cosubstrate GTP, pH 8.1, 30°C | Brevibacillus brevis | |
0.77 | - |
GTP | 30°C, pH 8.1 | Brevibacillus brevis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required as a counter-ion for the nucleoside substrate, excess of Mg2+ is inhibitory | Brevibacillus brevis | |
Mn2+ | can partially replace Mg2+ in activation | Brevibacillus brevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
sucrose density gradient centrifugation | Brevibacillus brevis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacillus brevis | - |
ATCC 8185 | - |
Purification (Comment) | Organism |
---|---|
- |
Brevibacillus brevis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Brevibacillus brevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-methyl-ATP + GTP | - |
Brevibacillus brevis | 2'-methyl-AMP + guanosine 3'-diphosphate 5'-triphosphate | - |
? | |
8-bromo-ATP + GTP | - |
Brevibacillus brevis | 8-bromo-AMP + guanosine 3'-diphosphate 5'-triphosphate | - |
? | |
ATP + GDP | preferred substrate | Brevibacillus brevis | AMP + guanosine 3'-diphosphate 5'-diphosphate | i.e ppGpp | ? | |
ATP + GTP | - |
Brevibacillus brevis | AMP + guanosine 3'-diphosphate 5'-triphosphate | i.e. pppGpp | ? | |
ATP + guanosine 5'-tetraphosphate | - |
Brevibacillus brevis | AMP + guanosine 3'-diphosphate 5'-tetraphosphate | - |
? | |
ATP + ITP | - |
Brevibacillus brevis | AMP + inosine 3'-diphosphate 5'-triphosphate | - |
? | |
dATP + GTP | - |
Brevibacillus brevis | dAMP + guanosine 3'-diphosphate 5'-triphosphate | - |
? | |
additional information | no substrate: 5'(beta,gamma-imino)triphosphate, 1,N6-ethyladenosine triphosphate, no diphosphate acceptors: ATP, UTP, CTP, dGTP, dGDP, 2'-O-methyl-GDP, 7-methyl-GDP | Brevibacillus brevis | ? | - |
? |