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Literature summary for 2.7.6.3 extracted from

  • Kasekarn, W.; Sirawaraporn, R.; Chahomchuen, T.; Cowman, A.F.; Sirawaraporn, W.
    Molecular characterization of bifunctional hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase from Plasmodium falciparum (2004), Mol. Biochem. Parasitol., 137, 43-53.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
Guanidine-HCl 0.25 M, 50% inhibitioin Plasmodium falciparum
KCl activation at 0.4 M, inhibition at higher concentration Plasmodium falciparum
NaCl activation at 0.2 M, inhibition at higher concentration Plasmodium falciparum
sulfadoxine competitive, moderately potent Plasmodium falciparum
sulfathiazole competitive Plasmodium falciparum
Urea 0.9 M, 50% inhibition Plasmodium falciparum

Metals/Ions

Metals/Ions Comment Organism Structure
KCl activation at 0.4 M, inhibition at higher concentration Plasmodium falciparum
Mg2+ required Plasmodium falciparum
NaCl activation at 0.2 M, inhibition at higher concentration Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
4 * 83000, SDS-PAGE Plasmodium falciparum
330000
-
gel filtration Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Plasmodium falciparum

Storage Stability

Storage Stability Organism
-20°C or -80°C for 3 months in 20 mM Tris-HCl, pH 8.0 buffer containing 10 or 20% glycerol, no loss of activity. In buffer without glycerol, 30 and 50% of the enzyme activity are lost when stored for 6 months at -80°C and -20°C, respectively Plasmodium falciparum

Subunits

Subunits Comment Organism
tetramer 4 * 83000, SDS-PAGE Plasmodium falciparum

Synonyms

Synonyms Comment Organism
hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase bifunctional enzyme Plasmodium falciparum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 45
-
Plasmodium falciparum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 45 30°C: about 40% of maximal activity, 37-45°C: optimum Plasmodium falciparum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
complete inactivation Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Plasmodium falciparum

pH Range

pH Minimum pH Maximum Comment Organism
8.5 10.5 pH 8.5: about 40% of maximal activity, pH 10.5: about 90% of maximal activity Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.011
-
sulfadoxine
-
Plasmodium falciparum

pI Value

Organism Comment pI Value Maximum pI Value
Plasmodium falciparum calulation from amino acid sequence
-
7.1