Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
complexed with thiamin diphosphate-Mg2+ or thiamin-Mg2+, hanging drop vapor diffusion method, using 1.15-1.3 M sodium citrate, 0.1 M Na-HEPES, pH 8.6, at 22°C | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel-chelate chromatography | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + thiamine | - |
Mus musculus | AMP + thiamine diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | x-ray crystallography | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
thiamin pyrophosphokinase | - |
Mus musculus |
TPK | - |
Mus musculus |