Literature summary for 2.7.6.2 extracted from

Santini, S.; Monchois, V.; Mouz, N.; Sigoillot, C.; Rousselle, T.; Claverie, J.; Abergel, C.
Structural characterization of CA1462, the Candida albicans thiamine pyrophosphokinase (2008), BMC Struct. Biol., 8, 33.

Cloned(Commentary)

Cloned (Comment)	Organism
gene CA1462, expression in Escherichia coli	Candida albicans

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme in complex with ATP analogue AMP-PNP and thiamine, hanging drop vapor diffusion method, mixing of 500 nl of protein solution with 500 nl of reservoir solution containing 17.5-20% PEG 4000, 0.2 M MgCl2 , 0.1 M Tris, and 20% glycerol, pH 7.0-pH 7.5, and equilibration against 1 ml or reservoir solution, X-ray diffraction structure determination and analysis at 1.96-2.1 A resolution	Candida albicans

Crystallization (Comment)

Organism

purified recombinant enzyme in complex with ATP analogue AMP-PNP and thiamine, hanging drop vapor diffusion method, mixing of 500 nl of protein solution with 500 nl of reservoir solution containing 17.5-20% PEG 4000, 0.2 M MgCl2 , 0.1 M Tris, and 20% glycerol, pH 7.0-pH 7.5, and equilibration against 1 ml or reservoir solution, X-ray diffraction structure determination and analysis at 1.96-2.1 A resolution

Candida albicans

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	ligands electronic density map and Mg2+ coordination structure, the delta-oxygen from D113 and D115 and one oxygen atom from each phosphate group of the thiamine-PNP. The last coordination of this Mg2+ involves an oxygen atom from the free inorganic phosphate, overview	Candida albicans
additional information	ligand binding structures, overview	Candida albicans

Organism

Organism	UniProt	Comment	Textmining
Candida albicans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant CA1462 from Escherichia coli	Candida albicans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.000367	-	recombinant enzyme	Candida albicans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	thiamine binding site structure, overview	Candida albicans	?	-	?

Subunits

Subunits	Comment	Organism
dimer	three-dimensional enzyme structure, modelling, overview	Candida albicans

Synonyms

Synonyms	Comment	Organism
CA1462	-	Candida albicans
thiamine pyrophosphokinase	-	Candida albicans
TPK	-	Candida albicans