Application | Comment | Organism |
---|---|---|
medicine | Malaria is a devastating and quite often deadly parasitic disease, resulting in significant public health problems in the tropics. The inevitable emergence of drug-resistant forms requires the continuous discovery and development of new antimalarials. These drugs should only target the parasite, with only harmless or no effects on the human host. Thus, ideal drug targets would be peculiarities in the parasite metabolism, such as vitamin B1 and B6 biosyntheses that are absent in its host. | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.062 | - |
pyrithiamine | - |
Plasmodium falciparum | |
0.073 | - |
thiamine | thiamine monophosphate is not accepted | Plasmodium falciparum | |
1.1 | - |
ATP | - |
Plasmodium falciparum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Optimal activity obtained by addition of 2-4 mM Mg2+. | Plasmodium falciparum | |
Mn2+ | Substitution of magnesium by Mn2+ decreases the enzyme activity by 42%. | Plasmodium falciparum | |
Zn2+ | Substitution of magnesium by Zn2+ decreases the enzyme activity by 85%. | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
for PfTPK3, SDS-PAGE analysis followed by Western blotting | Plasmodium falciparum |
43000 | - |
for PfTPK2, SDS-PAGE analysis followed by Western blotting | Plasmodium falciparum |
47000 | - |
for PfTPK, SDS-PAGE analysis followed by Western blotting | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiamine + ATP | Saccharomyces cerevisiae | - |
thiamine diphosphate + AMP | - |
? | |
thiamine + ATP | Schizosaccharomyces pombe | - |
thiamine diphosphate + AMP | - |
? | |
thiamine + ATP | Plasmodium falciparum | - |
thiamine diphosphate + AMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Schizosaccharomyces pombe | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.009 | - |
PfTPK3, with 4 mM magnesium ions in the enzyme assay | Plasmodium falciparum |
0.017 | - |
PfTPK2, with 4 mM magnesium ions in the enzyme assay | Plasmodium falciparum |
0.017 | - |
substrate: pyrithiamine, PfTPK | Plasmodium falciparum |
0.027 | - |
PfTPK, with 4 mM magnesium ions in the enzyme assay | Plasmodium falciparum |
999 | - |
no activity with thiochrome | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyrithiamine + ATP | its specific activity is 30% lower in comparison to its natural substrate thiamine | Plasmodium falciparum | pyrithiamine diphosphate + AMP | - |
? | |
thiamine + ATP | - |
Saccharomyces cerevisiae | thiamine diphosphate + AMP | - |
? | |
thiamine + ATP | - |
Schizosaccharomyces pombe | thiamine diphosphate + AMP | - |
? | |
thiamine + ATP | - |
Plasmodium falciparum | thiamine diphosphate + AMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | gel filtration using a calibrated Superdex S-200 column | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
Plasmodium falciparum thiamine pyrophosphokinase | - |
Plasmodium falciparum |
pyrophosphokinase | - |
Saccharomyces cerevisiae |
pyrophosphokinase | - |
Schizosaccharomyces pombe |
pyrophosphokinase | - |
Plasmodium falciparum |
thiamine pyrophosphokinase | - |
Saccharomyces cerevisiae |
thiamine pyrophosphokinase | - |
Schizosaccharomyces pombe |
thiamine pyrophosphokinase | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
PfTPK | Plasmodium falciparum |