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Literature summary for 2.7.6.2 extracted from

  • Molin, W.T.; Fites, R.C.
    Isolation and characterization of thiamin pyrophosphotransferase from Glycine max seedlings (1980), Plant Physiol., 66, 308-312.
    View publication on PubMed
Search for more literature for 2.7.6.2

Inhibitors

Inhibitors Comment Organism Structure
AMP 0.5 mM, 8% inhibition Glycine max
diphosphate 0.5 mM, only 3% inhibition Glycine max
oxythiamine 0.5 mM; 4% inhibition Glycine max
PCMB 0.5 mM, 36% inhibition Glycine max
pyrithiamine 0.5 mM, 77% inhibition Glycine max
thiamine diphosphate 0.5 mM; 95% inhibition Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00464
-
 thiamine pH 8.1, 37°C Glycine max
2.6
-
 GTP pH 8.1, 37°C Glycine max
7.6
-
 CTP pH 8.1, 37°C Glycine max
11.2
-
 UTP pH 8.1, 37°C Glycine max
18.3
-
 ATP pH 8.1, 37°C Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 gel filtration Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000061
-
-
 Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + thiamine
-
 Glycine max AMP + thiamine diphosphate
-
 ?
CTP + thiamine
-
 Glycine max CMP + thiamine diphosphate
-
 ?
GTP + thiamine
-
 Glycine max GMP + thiamine diphosphate
-
 ?
UTP + thiamine
-
 Glycine max UMP + thiamine diphosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1 7.3
-
 Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
6.4 8 pH 6.4: about 40% of maximal activity, pH 8.0: about 50% of maximal activity Glycine max