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Literature summary for 2.7.6.2 extracted from
- Partial purification and properties of thiamine pyrophosphokinase from pig brain (1975), Biochim. Biophys. Acta, 397, 377-394.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Butylthiamine | competitive with respect to thiamine | Sus scrofa |  |
| ethylthiamine | competitive with respect to thiamine | Sus scrofa | |
| oxythiamine | competitive with respect to thiamine | Sus scrofa | |
| pyrithiamine | competitive with respect to thiamine | Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0041 | - |
ATP | pH 7.8, 37°C | Sus scrofa | |
| 0.059 | - |
ATP | pH 7.8, 37°C | Sus scrofa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | for optimal activity the ratio of magnesium to ATP must be fixed at 0.6. The Mg(ATP)26- species might by the actual diphosphoryl donor | Sus scrofa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Sus scrofa |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + thiamine = AMP + thiamine diphosphate | partial ping pong mechanism | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00165 | - |
- |
Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + thiamine | - |
Sus scrofa | AMP + thiamine diphosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | 9.3 | in 0.028 M phosphate/glycylglycine buffer | Sus scrofa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 9.3 | pH 6.8: about 65% of maximal activity, pH 8.3-9.3: optimum | Sus scrofa |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Sus scrofa | isoelectric focusing | - |
4.2 |
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