Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Butylthiamine | competitive with respect to thiamine | Sus scrofa | |
ethylthiamine | competitive with respect to thiamine | Sus scrofa | |
oxythiamine | competitive with respect to thiamine | Sus scrofa | |
pyrithiamine | competitive with respect to thiamine | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0041 | - |
ATP | pH 7.8, 37°C | Sus scrofa | |
0.059 | - |
ATP | pH 7.8, 37°C | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | for optimal activity the ratio of magnesium to ATP must be fixed at 0.6. The Mg(ATP)26- species might by the actual diphosphoryl donor | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Sus scrofa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + thiamine = AMP + thiamine diphosphate | partial ping pong mechanism | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00165 | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + thiamine | - |
Sus scrofa | AMP + thiamine diphosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | 9.3 | in 0.028 M phosphate/glycylglycine buffer | Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 9.3 | pH 6.8: about 65% of maximal activity, pH 8.3-9.3: optimum | Sus scrofa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sus scrofa | isoelectric focusing | - |
4.2 |