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Literature summary for 2.7.6.2 extracted from

  • Mitsuda, H.; Takii, Y.; Iwami, K.; Yasumoto, K.; Nakajima, K.
    Enzymatic formation of thiamine pyrophosphate in plants (1979), Methods Enzymol., 62, 107-111.
    View publication on PubMed
Search for more literature for 2.7.6.2

Inhibitors

Inhibitors Comment Organism Structure
diphosphate 10 mM, complete inhibition Petroselinum crispum
iodoacetamide 10 mM Petroselinum crispum
KCN little effect Petroselinum crispum
molybdate little effect Petroselinum crispum
NaF little effect Petroselinum crispum
NEM
-
 Petroselinum crispum
PCMB 0.1 mM, complete inhibition, completely restored by 10 mM dithiothreitol Petroselinum crispum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00015
-
 thiamine
-
 Petroselinum crispum
0.8
-
 ATP
-
 Petroselinum crispum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 gel filtration Petroselinum crispum

Organism

Organism UniProt Comment Textmining
Petroselinum crispum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Petroselinum crispum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000722
-
-
 Petroselinum crispum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + thiamine
-
 Petroselinum crispum AMP + thiamine diphosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9 in Tris buffer Petroselinum crispum

pI Value

Organism Comment pI Value Maximum pI Value
Petroselinum crispum isoelectric focusing
-
 4.5