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Literature summary for 2.7.6.2 extracted from

  • Mitsuda, H.; Takii, Y.; Iwami, K.; Yasumoto, K.
    Mechanism and regulation of thiamine pyrophosphokinase from parsely leaf (1975), J. Nutr. Sci. Vitaminol., 21, 189-198.
    View publication on PubMed
Search for more literature for 2.7.6.2

Inhibitors

Inhibitors Comment Organism Structure
diphosphate competitive with respect to thiamine Petroselinum crispum
Thiamine monophosphate
-
 Petroselinum crispum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.9
-
 thiamine
-
 Petroselinum crispum
4.5
-
 ATP ATP in form of MgATP2- Petroselinum crispum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ absolute requirement for a divalent cation such as Mg2+, Mn2+ or Co2+. In presence of 2 mM ATP maximal activity occurs with 1 mM Co2+ Petroselinum crispum
Mg2+ absolute requirement for a divalent cation such as Mg2+, Mn2+ or Co2+. In presence of 2 mM ATP maximal activity occurs with above 3 mM Mg2+ Petroselinum crispum
Mn2+ absolute requirement for a divalent cation such as Mg2+, Mn2+ or Co2+. In presence of 2 mM ATP maximal activity occurs with 0.5 mM Mn2+ Petroselinum crispum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 gel filtration Petroselinum crispum

Organism

Organism UniProt Comment Textmining
Petroselinum crispum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Petroselinum crispum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + thiamine
-
 Petroselinum crispum AMP + thiamine diphosphate
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2
-
 Thiamine monophosphate
-
 Petroselinum crispum
2.8
-
 diphosphate
-
 Petroselinum crispum