Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | required, maximal activity at 50 mM, slowly reduced to 58% at 120 mM and 17% at 200 mM | [Bacillus] caldolyticus | |
SO42- | can replace phosphate at about 30% of the maximal activity at a concentration of 0.5 M | [Bacillus] caldolyticus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | [Bacillus] caldolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | at low concentrations ADP inhibition is linearly competitive with respect to ATP. Less than 1% residual activity in presence of 5 mM ADP | [Bacillus] caldolyticus | |
GDP | 5 mM, less than 1% residual activity | [Bacillus] caldolyticus | |
SO42- | 1 M, strong inhibition | [Bacillus] caldolyticus | |
UTP | 5.0 mM, significant inhibition | [Bacillus] caldolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
ATP | - |
[Bacillus] caldolyticus | |
0.53 | - |
D-ribose 5-phosphate | - |
[Bacillus] caldolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | activity is 5-10% of the activity with Mg2+ | [Bacillus] caldolyticus | |
Cu2+ | activity is 5-10% of the activity with Mg2+ | [Bacillus] caldolyticus | |
Mg2+ | free Mg2+ required for maximal activity | [Bacillus] caldolyticus | |
Mn2+ | substitutes for Mg2+ at 30% of the activity obtained with Mg2+ | [Bacillus] caldolyticus | |
Zn2+ | activity is 5-10% of the activity with Mg2+ | [Bacillus] caldolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34497 | - |
6 * 34497, MALDI-TOF | [Bacillus] caldolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Bacillus] caldolyticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
[Bacillus] caldolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
440 | - |
- |
[Bacillus] caldolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | - |
[Bacillus] caldolyticus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 34497, MALDI-TOF | [Bacillus] caldolyticus |
Synonyms | Comment | Organism |
---|---|---|
5-phospho-alpha D-ribosyl 1-diphosphate synthase | - |
[Bacillus] caldolyticus |
PRibPP synthase | - |
[Bacillus] caldolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 65 | - |
[Bacillus] caldolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
half-life: 26 min | [Bacillus] caldolyticus |
65 | - |
half-life: 60 s | [Bacillus] caldolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | 8.8 | - |
[Bacillus] caldolyticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | pH 6.5: about 25% of maximal activity, pH 9.5: about 80% of maximal activity | [Bacillus] caldolyticus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.113 | - |
ADP | - |
[Bacillus] caldolyticus | |
0.49 | - |
GDP | - |
[Bacillus] caldolyticus |